Flow cytometry reagents and buffers

The effectiveness of flow cytometry is heavily dependent on the selection and use of various reagents and buffers, which are essential for sample preparation, staining, and analysis. Staining reagents, such as fluorescently labeled antibodies, target specific proteins or antigens on cell surfaces or within cells, allowing for precise identification and differentiation of cell types based on their expression profiles. Buffers are crucial in maintaining optimal conditions during the process; they preserve cell viability, minimize non-specific binding, and stabilize cellular structures.

Additionally, specialized reagents like viability dyes help distinguish live cells from dead ones, enhancing the accuracy of results. By carefully optimizing these reagents and buffers, researchers can ensure that flow cytometry remains a vital tool for exploring complex biological systems and advancing our understanding of cellular behavior.