Phospho Flow Analysis

Flow cytometric analysis of phosphorylated proteins using phospho-specific antibodies

Phospho Flow Analysis

Cells often use phosphylation to transduces extracellular singals (e.g. cytokines) to intracellular changes in cell physiology. Flow cytometric analysis of phosphorylated proteins using phospho-specific antibodies allows researchers a quick and effective way to measure signaling cascades in individual cells. Traditional immunofluorescent cell-surface staining and phospho flow cytometry protocols can also be combined for simultaneous analysis of surface molecules and intracellular phosphorylated signaling proteins.