1-step SYBR Green qRT-PCR

1-step SYBR Green qRT-PCR

1-step qRT-PCR Protocol on Bio-Rad iCycler™

This protocol is intended for use with the Bio-Rad iCycler iQ®, iQ™5, and MyiQ™ real-time PCR detection systems.

1-step qRT-PCR Protocol for ABI Prism™

This protocol is intended for use with the ABI PRISM® 7000, 7700, 7900HT, ABI 7300 Real-Time PCR Systems, GeneAmp® 5700, StepOne™, StepOnePlus™, and ViiA™ 7. This kit is not compatible with instruments that use ROX at a final concentration lower than 500 nM.

1-step qRT-PCR Protocol for LightCycler® 480

This protocol is intended for use with the Roche LightCycler® 480 real-time PCR instrument.