Protease assay kits
For drug target screening and discovery
We are pleased to spotlight our wide portfolio of iD SensoLyte® Protease Activity Assay kits for the study of proteases having roles in specific diseases. Further, our Custom iD Optimization services caters to custom assay development with high quality and minimal lead time.
Catalog&custom protease activity assays - Features&benefits
- Ready-to-use mix and read homogeneous format ideal for HTS.
- All essential components for performing assays in 96-well plate formats.
- Detailed protocols and references included, ensuring value and reliability.
- Proprietary substrates (FRET pairs) in many of our protease kits.
- Custom assay design and development offering a full spectrum of dyes and quenchers covering all detection channels.
SensoLyte® Catalog Protease Kits&Related Services
- Targeted Catalog Assay Kits: iD SensoLyte® catalog Protease Assay kits feature proteases that have important roles in
- Neuroscience (Alzheimer's, Parkinson's MS),
- Cancer,
- Epigenetics,
- Cell adhesion&ECM (MMPs),
- Cardiovascular/Metabolic and
- Viral pathogenesis.
- Bulk FRET or fluorogenic substrates: For developing your own HTS assays, we conveniently offer substrates separately from our assay kits.
- iD Optimization and Screening: includes optimizing and scale up of any SensoLyte® assay kit, and small molecules screening with choice of assay kit.
SensoLyte® Catalog Protease Assay Kits
Neuroscience
Type
Product
Product code
Secretase
SensoLyte® 520 β - Secretase Assay Kit *Fluorimetric*
SensoLyte® 520 TACE (α - Secretase) Activity Assay Kit
SensoLyte® 520 BACE2 Activity Assay *Fluorimetric*
SensoLyte® 520 TACE (α - Secretase) Activity Assay Kit
SensoLyte® 520 BACE2 Activity Assay *Fluorimetric*
AS-71144
AS-72085
AS-72225
AS-72085
AS-72225
Calpains
SensoLyte® AMC Calpain Activity Assay Kit *Fluorimetric*
SensoLyte® 520 Calpain Activity Assay Kit *Fluorimetric*
SensoLyte® 520 Calpain Activity Assay Kit *Fluorimetric*
AS-72150
AS-72149
AS-72149
Cell adhesion&ECM
Type
Product
Product code
Elastase
SensoLyte® Rh110 Elastase Assay Kit *Fluorimetric*
SensoLyte® Green Elastase Assay Kit *Fluorimetric*
SensoLyte® Green Elastase Assay Kit *Fluorimetric*
AS-72179
AS-72178
AS-72178
Cancer&Apoptosis
Type
Product
Product code
Cardiovascular&metabolic related
Type
Product
Product code
ADAMTS
SensoLyte® 520 ADAMTS13 Activity Assay, *Fluorimetric*
SensoLyte® 520 ADAM10 Activity Assay Kit *Fluorimetric*
SensoLyte® 520 ADAM10 Activity Assay Kit *Fluorimetric*
AS-72232
AS-72226
AS-72226
Factor Xa
SensoLyte® Rh110 Factor Xa Assay Kit *Fluorimetric*
SensoLyte® 520 Factor Xa Assay Kit *Fluorimetric*
SensoLyte® 520 Factor Xa Assay Kit *Fluorimetric*
AS-72207
AS-72208
AS-72208
Plasmin&Plasminogen
SensoLyte® AFC Plasmin Activity Assay Kit *Fluorimetric*
SensoLyte® Rh110 Plasmin Activity Assay Kit *Fluorimetric*
SensoLyte® Rh110 Plasmin Activity Assay Kit *Fluorimetric*
AS-72124
AS-72125
AS-72125
Renin
SensoLyte® 520 Renin Assay Kit *Fluorimetric*
SensoLyte® 390 Renin Assay Kit *Fluorimetric*
SensoLyte® 520 Rat Renin Assay Kit *Fluorimetric*
SensoLyte® 520 Mouse Renin Assay Kit *Fluorimetric*
SensoLyte® 390 Renin Assay Kit *Fluorimetric*
SensoLyte® 520 Rat Renin Assay Kit *Fluorimetric*
SensoLyte® 520 Mouse Renin Assay Kit *Fluorimetric*
AS-72040
AS-72039
AS-72140
AS-72161
AS-72039
AS-72140
AS-72161
Thrombin
SensoLyte® 520 Thrombin Activity Assay Kit *Fluorimetric*
SensoLyte® AFC Thrombin Activity Assay Kit *Fluorimetric*
SensoLyte® AFC Thrombin Activity Assay Kit *Fluorimetric*
AS-72129
AS-72130
AS-72130
DPPIV
AS-72196
AS-72197
AS-72197
Enterokinase
SensoLyte® 520 Enterokinase Activity Assay Kit *Fluorimetric*
SensoLyte® Rh110 Enterokinase Activity Assay Kit *Fluorimetric*
SensoLyte® Rh110 Enterokinase Activity Assay Kit *Fluorimetric*
AS-72209
AS-72210
AS-72210
Epigenetics
Type
Product
Product code
Enterokinase
SensoLyte® 520 Enterokinase Activity Assay Kit *Fluorimetric*
SensoLyte® Rh110 Enterokinase Activity Assay Kit *Fluorimetric*
SensoLyte® Rh110 Enterokinase Activity Assay Kit *Fluorimetric*
AS-72209
AS-72210
AS-72210
Viral&bacterial proteases
Type
Product
Product code
HCV Protease
SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric*
SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric*
SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric*
SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric*
SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric*
AS-72087
AS-71126
AS-71145
AS-71126
AS-71145
WNV Protease
SensoLyte® 440 West Nile Virus Protease Assay Kit *Fluorimetric*
SensoLyte® 570 West Nile Virus Protease Assay Kit *Fluorimetric*
SensoLyte® 570 West Nile Virus Protease Assay Kit *Fluorimetric*
AS-72079
AS-72080
AS-72080
Role of Proteases
Proteases are enzymes that catalyze the hydrolysis of peptide and isopeptide bonds that link amino acids within proteins, in the process referred to as proteolysis. Proteases have evolved as multifunctional enzymes adapting and diversifying to various biological roles resultant of changes in their expression patterns, substrate specificities and structure-function relationships.Their roles include,
- Activating cytokines and receptors for cell signaling.
- Cell death mediators and Antigen processors:
- Activation of Bid in apoptosis by Caspases-8, Calpains&Cathepsins B, L S, H, D, acting via the loops or interdomain linker regions.
- Processing antigens via the lysosomal system for presentation by MHC class II molecules, such as cathepsins and deubiquitinating enzymes.
- Ecdomomain processors by exposing cryptic sites, such as beta-secretase during amyloid precursor protein processing or Shedding various transmembrane proteins such as metalloproteases and cysteine proteases.
- ECM modulators by using exosites as effective substrate binding,such as thrombin, ADAMTS-4 and most MMPs.
- Targets for viral inhibitors to arrest viral proteases processing viral polyproteins in causing pathogenicity.
Figure 2.Schematic representation of diverse roles of Proteases.