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Target Molecule Corp. (TargetMol) is a global high-tech enterprise, specializing in chemical and biological research products and service to meet the research needs of global customers. TargetMol has evolved into one of the biggest global small molecule suppliers with over 5000 inhibitors and 80+ compound libraries in stock. TargetMol offers a wide range of high quality research chemicals including novel life-science reagents, inhibitors, activator, APIs and natural compounds for laboratory and scientific use. Website : www.targetmol.com |
![Reducing agent - TCEP](https://www.clinisciences.com/de/upload/thumbs/chimie-bandeau-r4fmzm5v-hhqXkdpM-35d786.jpg)
Reducing agent - TCEP
TCEP (tris(2-carboxyethyl)phosphine) is a reducing agent frequently used in biochemical and molecular biology applications. TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis. It is soluble in water and available as a pH-neutral stabilized solution immobilized on an agarose support to facilitate the removal of the reducing agent.
Compared to the other two most commonly used reducing agents dithiothreitol (DTT) and β-mercaptoethanol (BME), TCEP has the advantages of being odorless, a more potent reducing agent, irreversible (in the sense that TCEP does not regenerate - the end product of disulfide cleavage by TCEP is actually two free thiols/cysteines), more hydrophilic and more resistant to oxidation in air.
TCEP is particularly useful for the labeling of cysteine residues with maleimides.
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