Biorbyt

Products for advancing your discoveries

 
From antibodies to proteins, biochemicals to ELISA kits, Biorbyt's high quality research products are available for you to enable world class research and contribution to a deeper understanding of life sciences.
 
A comprehensive catalog of life science research products covering the areas of Neuroscience, Signal transduction, Cell Biology, Cancer Metabolism, Cardiovascular and others
 
Product range :
 
  • Antibodies
Choose from over 100,000 primary antibodies and over 2,000 secondary antibodies. Monoclonals and polyclonals tested in a number of applications - WB, ELISA, IHC, FACS and many more.
 
  • Proteins
Choose from over 7,000 proteins and over 400 active proteins. All of our proteins have been validated for use in ELISAs, WB and IHC-P
 
  • Biochemicals
We have over 10,000 biochemicals for use in your research.
 
  • ELISA kits
We have over 2,000 ELISA kits covering many research areas . Each kit comes with a fully detailed protocol and examples of expected results.
 
 
Protein A Affinity resins

Protein A Affinity resins

Protein A is a cell wall protein from Staphylococcus aureus with a molecular weight between 35-50 kDa and shows high specificity for the Fc region of immunoglobulin molecules of many mammalian species. It is encoded by the spa gene, and its regulation is carried out at the level of the DNA topology, as a function of cellular osmolarity, by a two-component system called ArlS-ArlR. The quality of the resin (Protein A Agarose or equivalent) and stability of the immobilisation is important to avoid leakage of Protein A during the elution procedure.
Protein A affinity chromatography is a rapid one-step purification, which removes most nonIgG contaminants and can achieve purities close to homogeneity. It is particularly useful for purifications of tissue culture supernatant, where 10-100 fold concentrations can be achieved.

 Ni-NTA-coordination
Specifications:
  • Resin: Protein A Agarose F
  • Base Matrix: Agarose
  • Protein A capacity (hIgG): 30 mg (per 1 ml resin)

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