Biorbyt
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Products for advancing your discoveries From antibodies to proteins, biochemicals to ELISA kits, Biorbyt's high quality research products are available for you to enable world class research and contribution to a deeper understanding of life sciences.
A comprehensive catalog of life science research products covering the areas of Neuroscience, Signal transduction, Cell Biology, Cancer Metabolism, Cardiovascular and others
Product range :
Choose from over 100,000 primary antibodies and over 2,000 secondary antibodies. Monoclonals and polyclonals tested in a number of applications - WB, ELISA, IHC, FACS and many more.
Choose from over 7,000 proteins and over 400 active proteins. All of our proteins have been validated for use in ELISAs, WB and IHC-P
We have over 10,000 biochemicals for use in your research.
We have over 2,000 ELISA kits covering many research areas . Each kit comes with a fully detailed protocol and examples of expected results.
Website : https://www.biorbyt.com/
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NGS Library preparation
Next Generation Sequencing (NGS) requires careful preparation of the genetic material prior to the sequencing reaction to ensure reliable results.
The preparation of DNA banks is composed of 4 main steps whose sequence may vary depending on the methods used:
1. Fragmentation
DNA is fragmented either enzymatically or by sonication to create small random fragments.
2. End repair
Since DNA fragmentation does not result in homogeneous, open-ended fragments, end repair is necessary to ensure that each molecule is free of overhangs and contains 5' phosphate and 3' hydroxyl groups.
3. Binding of adapters
The adapters (small sequences of double-stranded synthetic DNA) are then bound to these fragments with the help of a DNA ligase. The adaptors allow the sequence to bind to the support but also to identify the sample analyzed thanks to the indexes. The adapters contain motifs necessary for the following steps (clonal amplification and sequencing)
4. Amplification of the bank
Amplification of the bank is necessary so that the signal received by the sequencer is strong enough to be detected accurately.
For the preparation of RNA libraries, a reverse transcription step must be added in order to obtain cDNA libraries.
