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> Anti-Centromere Protein Antibody

Anti-Centromere Protein Antibody

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Katalog-Nummer 15-234

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Anti-Centromere Protein
A microscopy image of a cell from BJ hTERT cell line treated with aphidicolin to induce genomic instability. Immunolabeling of the cell positively labelling CREST/Centromere Protein (red, 1:500, Cat No. 15-234) and labelling DNA with DAPI. The anaphase cell has a segregation error which lacks centromeric staining unlike the mass of properly segregating chromosomes. Image kindly provided by Nadeem Shaikh and Sarah McClelland from Barts Cancer Institute, Queen Mary University of London. Confocal image of kinetochore-microtubule attachments in mouse control (CON) and IMQ treated oocytes where kinetochores are dyed purple by CREST antibody, microtubules are stained green by the anti-tubulin antibody. Chromosomes are dyed blue by Hoechst 33342.Image from publication CC-BY-4.0. PMID: 35589679Immunofluorescence showing SCP3 (green) and the co-localization of VCL (red) and CREST-labeling centromeres (blue) on squashed mouse spermatocytes. Image from publication CC-BY-4.0. PMID: 35805097Immunofluorescence of control and nivalenol (NIV) treated mouse oocytes where α-tubulin is labeled green, centromeres are labeled red and DNA is dyed blue by Hoechst. Image from publication CC-BY-4.0. PMID: 35746834Immunostaining showing that there is no difference for centromere intensity between mouse WT and Fbxw24-KO germ cells, meanwhile SYCP3 intensity significantly increased in Fbxw24-KO germ cells. DNA in blue, SYCP3 in red, centromere in green. Image from publication CC-BY-4.0. PMID: 35858239Representative maximum z-projections of in situ chromosome spreads from a mouse euploid egg (kinetochores (CREST), green or gray scale) (DNA, blue). Image from publication CC-BY-4.0. PMID: 35810327Immunofluorescence of HeLa Kyoto cells transiently expressing different Sgo1-GFP constructs (Sgo1-GFP, Sgo1Nmut-GFP, Sgo14A-GFP, or Sgo1Nmut/4A-GFP) and depleted of endogenous Sgo1 using siRNA oligonucleotides showing endogenous Borealin and ACA. DAPI was used for DNA staining. Image from publication CC-BY-4.0. PMID: 35776132Uninjected, Control, CBS-KD human oocytes cultured in M16 medium for 8 h to MI for immunofluorescence with Mitotic arrest deficiency 1 (MAD1) (red) and human anti-centromere antibody (CREST) (green). Image from publication CC-BY-4.0. PMID: 36053797Human oocytes in Uninjected, Control, CBS-KD groups were cultured in M16 medium for 8 h to MI and then incubated on ice for 5 min and processed for immunofluorescence with acetylated α-tubulin (red) and CREST antibody (green). Image from publication CC-BY-4.0. PMID: 36053797Representative image of RPE1 cell with an acentric micronucleus. CREST antibody was used to stain for presence of centromeric proteins. Image from publication CC-BY-4.0. PMID:36266663Immunofluorescence images of porcine oocytes shortly after NEBD (z-projections, example 1: 14 sections, example 2: 11 sections every 0.19 µm) and after chromosome cluster formation (z-projection, 14 sections every 0.19 µm). F-actin (phalloidin; white); kinetochores (ACA)(cat. 15-234, 1:250; cyan); DNA (Hoechst, magenta,). Image from publication CC-BY-4.0. PMID:36732633Control and Pak2-KD metaphase mouse oocytes were labeled with CREST antibody for kinetochores (cat. 15-234, 1:300; purple), anti-tubulin antibody for microtubules (green), and Hoechst 33342 for chromosomes (blue). Image from publication CC-BY-4.0. PMID:36813765Representative images of the morphology of K-M attachments in MI oocytes from both control and BBP-exposed group. α-tubulin (gray); CREST (cat. 15-234, 1:200; red); DNA (blue). Image from publication CC-BY-4.0. PMID:36756972Localization of telomeric sequences in Thylamys elegans and Macropus eugenii. Spread spermatocytes were labeled with antibodies against SYCP3 (blue), centromeric proteins (cat. 15-234, 1:50; red) and telomeric sequences (green) in (D) and (H). Image from publication CC-BY-4.0. PMID:37181752Costaining of chromosomes (DAPI, blue) and centromeres (cat. 15-234, 1:250; green) in representative nuclei of metaphase short-term cultures, established from Nf2KO-Setd2KO-Trp53KO-4q9p21 tumor-bearing mice. Image from publication CC-BY-4.0. PMID:37365326Representative immunofluorescence images of RioK1 at kinetochores (CREST) (cat. 15-234, 1:50) in prometaphase and metaphase cells. Image from publication CC-BY-4.0. PMID:37263996Confocal imaging and quantification of Plk1 signal intensities at centrosomes and kinetochores (cat. 15-234) after treating HeLa cells with DMSO or 10 μM of Allopole for 2 h. Arrowheads, centrosomes; arrows, misaligned chromosomes. Signal intensities were quantified from both centrosome signals and randomly chosen three to four kinetochore signals per cell for a total of greater than or equal to 18 cells/experiment. Image from publication CC-BY-4.0. PMID:37603740Pachytene and pachytene-like spermatocytes of A. maximowiczii, MAX (A–C), A. evoronensis “Argi” × A. evoronensis “Evoron”, EVA × EVE (D–F), A. mujanensis × A. maximowiczii, MUJ × MAX (G–I), A. evoronensis “Argi” × A. mujanensis, EVA × MUJ (J–L), and A. evoronensis “Argi” × A. maximowiczii, EVA × MAX (M–O) after immunolocalization of SYCP3, SYCP1, MLH1, γH2A.X, and centromeric proteins (ACA, cat. 15-234, 1:100). Arrowheads show asynapsed SC regions of the autosomes. CC-BY-4.0. PMID:37263996Representative images of chromosome spreading (cat. 15-234, 1:200) of euploid and aneuploid mouse oocytes. Image from publication CC-BY-4.0. PMID:37386516RPE-1 cells expressing H2B-GFP and α-Tub-mRFP (magenta) were treated as indicated, fixed and stained for centromeres (ACA) (cat. 15-234, 1:400; white) and DNA (DAPI, blue). BI2536 was added at the IC30 concentration (5 nM). Representative cells are shown, with their measured spindle lengths and centromere dispersion ratios (r). Image from publication CC-BY-4.0. PMID:37639469RPE1 53BP1‐V5 cells were either left untreated (async = asynchronous) or synchronized in prometaphase in medium containing nocodazole, fixed immediately (M = mitosis) or after 2, 4, or 6 h and co‐stained with V5 and CREST (cat. 15-234, 1:500). Image from publication CC-BY-4.0. PMID:37888778C3a-peptide treatment does not induce chromosome aneuploidy of mouse oocytes. Representative images of MII oocyte immunofluorescence stained with DAPI (blue) and anti-CREST (cat. 15-234, 1:500; magenta) antibodies showing euploidy and aneuploidy in the oocytes, respectively (left). Image from publication CC-BY-4.0. PMID:38066646Pachytene spermatocytes of linnet (A), bullfinch (B), siskin (C), and goldfinch (D) after immunolocalization of SYCP3 (red), MLH1 (green) and centromere proteins (blue). Arrowheads indicate metapolycentromeres and GRC. Image from publication CC-BY-4.0. PMID:38066976Immunostaining with anti-centromere antibody ACA to show centromeres in detached chromosome ends in Zfp541 KO pachytene spermatocytes. Image from publication CC-BY-4.0. PMID:37921559

SKU: 15-234

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Citations (299)

Product Specific References for Applications and Species

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Immunocytochemistry: Kangaroo
PMID Dilution Publication
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Immunocytochemistry: Lizard
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Immunocytochemistry: Mouse
PMID Dilution Publication
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Immunocytochemistry: Porcine
PMID Dilution Publication
397157661:50Bellou, E, et al. 2024. Chromosome architecture and low cohesion bias acrocentric chromosomes towards aneuploidy during mammalian meiosis. Nature Communications, 10713.
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Immunocytochemistry: Rat
PMID Dilution Publication
346687191:25Begley, M.A., et al. 2021. K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15. Molecular Biology of the Cell, br11.
Immunocytochemistry: Snake
PMID Dilution Publication
371817521:50Valero-Regalón, F.J., et al. 2023. Divergent Patterns of Meiotic Double Strand Breaks and Synapsis Initiation Dynamics Suggest an Evolutionary Shift in the Meiosis Program Between American and Australian Marsupials. Frontiers in Cell and Developmental Biology, 1147610.
365524841:500Spangenberg, V., et al. 2022. Cytogenetic Analysis of the Bimodal Karyotype of the Common European Adder, Vipera berus (Viperidae). Animals, 3563.
Immunohistochemistry: Bird
PMID Dilution Publication
380669761:70Grishko, E, et al. 2023. Cytological Analysis of Crossover Frequency and Distribution in Male Meiosis of Cardueline Finches (Fringillidae, Aves). Animals, 3624.
349383171:50Poignet, M., et al. 2021. Comparison of karyotypes in two hybridizing passerine species: conserved chromosomal structure but divergence in centromeric repeats. Frontiers in Genetics, 768987.
Immunohistochemistry: Bovine
PMID Dilution Publication
36720222not listedWu, J., et al. 2023. Microtubule Nucleation from the Fibrous Corona by LIC1-pericentrin Promotes Chromosome Congression. Current Biology, .
339642101:250Cavazza, T., et al. 2021. Parental genome unification is highly error-prone in mammalian embryos. Cell, 2860-2877.
Immunohistochemistry: Fish
PMID Dilution Publication
387957381:100Lisachov, A, et al. 2024. Genome-wide sequence divergence of satellite DNA could underlie meiotic failure in male hybrids of bighead catfish and North African catfish (Clarias, Clariidae). Genomics, 110868.
Immunohistochemistry: Human
PMID Dilution Publication
390021331:500Hsu, J., et al. 2024. Protocol for iterative indirect immunofluorescence imaging in cultured cells, tissue sections, and metaphase chromosome spreads. STAR Protocols, 103190.
367326331:250Harasimov, K., et al. 2023. Actin-driven Chromosome Clustering Facilitates Fast and Complete Chromosome Capture in Mammalian Oocytes. Nature Cell Biology, 439-452.
354430321:1 (supe)Marcon, F., et al. 2022. The Mitochondrial Poison Carbonyl Cyanide 3-Chlorophenyl Hydrazone (CCCP) Induces Aneugenic Effects in Primary Human Fibroblasts: a Possible Link Between Mitochondrial Dysfunction and Chromosomal Loss. Mutagenesis, 155-163.
339642101:250Cavazza, T., et al. 2021. Parental genome unification is highly error-prone in mammalian embryos. Cell, 2860-2877.
Immunohistochemistry: Mouse
PMID Dilution Publication
398248111:50-1:100Kang, Z, et al. 2025. NKAPL facilitates transcription pause-release and bridges elongation to initiation during meiosis exit. Nature Communications, 791.
39019259not listedJin, Y., et al. 2024. MIB2 functions in oocyte meiosis by modulating chromatin configuration. Molecular & Cellular Proteomics, 100813.
390021331:500Hsu, J, et al. 2024. Protocol for iterative indirect immunofluorescence imaging in cultured cells, tissue sections, and metaphase chromosome spreads. STAR Protocols, 103190.
389602341:50Chang, H., et al. 2024. Fbxo28 is essential for spindle migration and morphology during mouse oocyte meiosis I. International Journal of Biological Macromolecules, 133232.
385537281:100Ju, JQ, et al. 2024. Kinesin KIFC3 is essential for microtubule stability and cytokinesis in oocyte meiosis. Cell Communication and Signaling, 199.
385460431:50Sun, SM, et al. 2024. Loss of UBE2S causes meiosis I arrest with normal spindle assembly checkpoint dynamics in mouse oocytes. Development, 0.
38244729not listedZhang, M., et al. 2024. Prothioconazole exposure disrupts oocyte maturation and fertilization by inducing mitochondrial dysfunction and apoptosis in mice. Free Radical Biology & Medicine, 274-284.
382185861:100Zhang, M., et al. 2024. Copper oxide nanoparticles impairs oocyte meiosis maturation by inducing mitochondrial dysfunction and oxidative stress. Food and Chemical Toxicology, 114441.
379215591:300Zhou, X, et al. 2023. ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression. Cell proliferation, e13567.
378382131:100Wang, W, et al. 2023. Alpha-lipoic acid supplementation reverses the declining quality of oocytes exposed to cyclophosphamide. Food and Chemical Toxicology, 114090.
37837697not listedZhang, Y, et al. 2023. Butylparaben weakens female fertility via causing oocyte meiotic arrest and fertilization failure in mice. Ecotoxicology and Environmental Safety, 115561.
374811221:150Zhang, H, et al. 2023. LSM14B Coordinates Protein Component Expression in the P-body and Controls Oocyte Maturation. Journal of genetics and genomics = Yi chuan xue bao, 0.
373865161:200Luo, YY, et al. 2023. Growth Hormone Reduces Aneuploidy and Improves Oocytes Quality by JAK2-MAPK3/1 Pathway in Aged Mice. Journal of translational medicine, 426.
373059661:500Cao, G, et al. 2023. PARP12 Regulates Mouse Oocyte Meiotic Maturation. Journal of Cellular Physiology, 1580-1591.
372393821:100Bikchurina, T, et al. 2023. Chromosome Asynapsis Is the Main Cause of Male Sterility in the Interspecies Hybrids of East Asian Voles (Alexandromys, Rodentia, Arvicolinae). Genes, 1022.
370124511:200Suebthawinkul, C., et al. 2023. Morphokinetic Parameters of Mouse Oocyte Meiotic Maturation and Cumulus Expansion are not Affected by Reproductive Age or Ploidy Status. Journal of Assisted Reproduction and Genetics, .
369897151:200Qu, J., et al. 2023. Near-infrared Fluorophore IR-61 Improves the Quality of Oocytes in Aged Mice via Mitochondrial Protection. Biomedicine & Pharmacotherapy, 114571.
Immunohistochemistry: Non-Human Primate
PMID Dilution Publication
347043421:30Blengini, C.S., et al. 2021. Age‐dependent integrity of the meiotic spindle assembly checkpoint in females requires Aurora kinase B. Aging Cell, e13489.
Immunohistochemistry: Porcine
PMID Dilution Publication
367326331:250Harasimov, K., et al. 2023. Actin-driven Chromosome Clustering Facilitates Fast and Complete Chromosome Capture in Mammalian Oocytes. Nature Cell Biology, 439-452.
Immunohistochemistry: Rat
PMID Dilution Publication
390021331:500Hsu, J, et al. 2024. Protocol for iterative indirect immunofluorescence imaging in cultured cells, tissue sections, and metaphase chromosome spreads. STAR Protocols, 103190.

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Picrosirius Red Stain Kit
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Tween 20
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