BRD4 TR-FRET, BioAssay™ Kit (BD1+BD2, Bromodomain, HUNK1, MCAP)

Katalog-Nummer 298369-1Kit

Size : 1Kit

Marke : US Biological

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298369 BRD4 TR-FRET, BioAssay™ Kit (BD1+BD2, Bromodomain, HUNK1, MCAP)

Clone Type
Polyclonal
Shipping Temp
Dry Ice
Storage Temp
RT/-20°C/-70°C

The BRD4 (BD1+BD2) TR-FRET BioAssay™ Kit is designed to measure the inhibition of BRD4 (BD1+ BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD4, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.||Applications: |Suitable for use in screening small molecular inhibitors for drug discovery and HTS applications. ||Kit Components:|298369A: Microtiter plate, 1x 1 plate|*298369B: BRD4 (BD1+BD2), 1x10ug |*298369C: BET Bromodomain Ligand, 1x50ul |*298369D: Non-acetylated Ligand 1, 1x15ul |**298369E: Tb-labeled donor, 2x10ul |**298369F: Dye-labeled acceptor, 2x10ul |**298369G: BRD TR-FRET Assay Buffer 1, 3X, 1x4ml ||Storage and Stability:|Store *298369B, *298369C and *298369D at -70°C. Store **298369E, **298369F and **298369G at -20°C. 298369A can be stored at RT. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
Filippakopoulos, P., et al., Cell 2012; 149:214.