Ccr1 (NM_009912) Mouse Tagged Lenti ORF Clone

Katalog-Nummer MR205412L4

Size : 10ug

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Ccr1 (NM_009912) Mouse Tagged Lenti ORF Clone

SKU
MR205412L4
Lenti ORF clone of Ccr1 (mGFP-tagged) - Mouse chemokine (C-C motif) receptor 1 (Ccr1)
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

    Click here to learn more.

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Specifications
Product Data
Target Symbol Ccr1
Synonyms Cmkbr1; Mip-1a-R
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR205412).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_009912
ORF Size 1065 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009912.2
RefSeq Size 2708 bp
RefSeq ORF 1068 bp
Locus ID 12768
UniProt ID P51675
Cytogenetics 9 75.05 cM
Summary Receptor for a C-C type chemokine. Binds to MIP-1-alpha, RANTES, and less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.[UniProtKB/Swiss-Prot Function]
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SKU Description Size
MC200995 Ccr1 (untagged) - Mouse chemokine (C-C motif) receptor 1 (Ccr1), (10ug) 10 ug
MG205412 Ccr1 (tGFP-tagged) - Mouse chemokine (C-C motif) receptor 1 (Ccr1) 10 ug
MR205412 Ccr1 (Myc-DDK-tagged) - Mouse chemokine (C-C motif) receptor 1 (Ccr1) 10 ug
MR205412L3 Lenti ORF clone of Ccr1 (Myc-DDK-tagged) - Mouse chemokine (C-C motif) receptor 1 (Ccr1) 10 ug
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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