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Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of human peripheral blood with CD45RA monoclonal antibody, clone HI100 (PerCP) (Cat # MAB13678).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against human CD45RA.

    Immunogen

    Fresh human lymph node lymphocytes depleted of surface immunoglobulin positive cells.

    Host

    Mouse

    Theoretical MW (kDa)

    220

    Reactivity

    Human

    Form

    Liquid

    Conjugation

    PerCP

    Purification

    Protein A/G purification

    Purity

    >90%

    Isotype

    IgG2b, kappa

    Recommend Usage

    Flow Cytometry (20 uL/106 cells)
    Immunofluorescence
    Immunohistochemistry (Frozen sections)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (protein stabilizer, 0.09% sodium azide).

    Storage Instruction

    Store in the dark at 4°C.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

    Immunofluorescence

    Flow Cytometry

    Flow cytometric analysis of human peripheral blood with CD45RA monoclonal antibody, clone HI100 (PerCP) (Cat # MAB13678).
  • Gene Info — PTPRC

    Entrez GeneID

    5788

    Protein Accession#

    P08575

    Gene Name

    PTPRC

    Gene Alias

    B220, CD45, CD45R, GP180, LCA, LY5, T200

    Gene Description

    protein tyrosine phosphatase, receptor type, C

    Omim ID

    126200 151460 609532

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq

    Other Designations

    CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|glycoprotein|leukocyte-common antigen|protein tyrosine phosphatase, receptor type, c polypeptide

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