Cell surface transferrin receptor (CD71) is a disulfide-bonded homodimeric glycoprotein. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. Transferrin receptor (CD71) has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.||Applications:|Suitable for use in Immunohistochemistry. Other applications not tested.||Rcommended Dilutions: |Immunohistochemistry (Formalin/paraffin): 1:20-1:40 for 20 minutes at RT. Staining of formalin-fixed tissues required boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Requires detection with a high-sensitivity detection system.|Optimal dilutions to be determined by the researcher.||Positive Control: |Placenta||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.