CHAPS

Biological Activity

CHAPS is a non-denaturing, zwitterionic detergent that dissolves membrane proteins. CHAPS are used to stabilize various protein-DNA complexes and can preserve the biochemical activity of proteins in solution. CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.

Chemical Information

Molecular Weight	614.88
Formula	C32H58N2O7S
CAS Number	75621-03-3
Solubility (25°C)	Water ≥ 50 mg/mL
Storage	2-8°C, dry, sealed

References

[1] P M Rodi, et al. Biochim Biophys Acta . Comparative study of the interaction of CHAPS and Triton X-100 with the erythrocyte membrane

[2] Ana Kroflic, et al. Langmuir. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence