Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot using CHK1 polyclonal antibody (Cat # PAB11360) shows detection of bands corresponding to CHK1 in Saccharomyces cerevisiae lysates.
Two strains of S.cerevisiae were tested. Lane 1 shows a predominant band at ~60 KDa. Lane 2 shows a predominant band at ~38 KDa.
Specific band staining is blocked when antibody is preincubated for 45 min at room temperature with 50 ug of peptide immunogen (lanes 3 and 4 respectively).
Lysates were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:750 in blocking buffer diluted 1:5 in PBS.
The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 minat room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of CHK1.

    Immunogen

    A synthetic peptide corresponding to amino acids 312-327 of Saccharomyces cerevisiae CHK1.

    Host

    Rabbit

    Reactivity

    Yeast

    Form

    Liquid

    Quality Control Testing

    Antibody Reactive Against Synthetic Peptide.

    Recommend Usage

    ELISA (1:2000-1:10000)
    Western Blot (1:200-1:2000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western blot using CHK1 polyclonal antibody (Cat # PAB11360) shows detection of bands corresponding to CHK1 in Saccharomyces cerevisiae lysates.
    Two strains of S.cerevisiae were tested. Lane 1 shows a predominant band at ~60 KDa. Lane 2 shows a predominant band at ~38 KDa.
    Specific band staining is blocked when antibody is preincubated for 45 min at room temperature with 50 ug of peptide immunogen (lanes 3 and 4 respectively).
    Lysates were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
    After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:750 in blocking buffer diluted 1:5 in PBS.
    The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 minat room temperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — CHK1

    Entrez GeneID

    852577

    Protein Accession#

    P38147;NP_009833

    Gene Name

    CHK1

    Gene Alias

    -

    Gene Description

    Chk1p

    Gene Ontology

    Hyperlink

    Gene Summary

    mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase

    Other Designations

    Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase

  • Publication Reference