Cytokeratin 18 (KRT18)(B23.1), 1mg/mL
Katalog-Nummer BNUM1263-50
Size : 50uL
Marke : Biotium
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Antibody number | #1263 |
|---|---|
| Antibody reactivity (target) | Cytokeratin 18 |
| Antibody type | Primary |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone | B23.1 |
| Isotype | IgG1, kappa |
| Molecular weight | 45 kDa |
| Synonyms | Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18 |
| Human gene symbol | KRT18 |
| Entrez gene ID | 3875 |
| SwissProt | P05783 |
| Unigene | 406013 |
| Immunogen | PMC-42 human breast carcinoma cells |
| Verified antibody applications | IHC (FFPE) (verified), WB (verified) |
| Antibody target cellular localization | Cytoskeleton |
| Species reactivity | Human. Does not react with Mouse, Rat, Sheep, Hamster, Cow, Dog or Pig. |
| Antibody application notes | Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Immunohistochemistry (formalin-fixed): 0.5-1 ug/mL for 30 min at RT, Flow cytometry: 0.5-1 ug/million cells, Western Blot 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user |
| Positive control | HCT116, MCF-7, HeLa, A431 cells. Breast Cancer. |
| Shipping condition | Room temperature |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
| Regulatory status | For research use only (RUO) |
| Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Cell/tissue expression | Epithelial cells |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
| Antibody research areas | Cancer, Cytoskeleton |
Product Description
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Catalog number key for antibody number 1263, Anti-Cytokeratin 18 (B23.1)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |