Dual Luciferase Reporter Gene Assay Kit
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- More Files
The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
- Specifications
Product Description
Dual Luciferase Reporter Gene Assay Kit is used for sequential measuring the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample.
Suitable Sample
Cell
Detection Method
Luminescence
Limit of Detection
2 fg luciferase
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard Curve
The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store the kit at -20°C.
- Applications
Functional Study
- Publication Reference
- Interference of KLF9 relieved the development of gestational diabetes mellitus by upregulating DDAH2.
Weixia Chen, Huiqin Wang, Jing Liu, Kaixia Li.
Bioengineered 2022 Jan; 13(1):395.
Application：Quant, Human, HTR8/Svneo cells.
- Exosome miR-134-5p restrains breast cancer progression via regulating PI3K/AKT pathway by targeting ARHGAP1.
Chao Yang, Geng Zhang, Yanshou Zhang, Shuo Zhang, Jingping Li, Yunjiang Liu.
The Journal of Obstetrics and Gynaecology Research 2021 Nov; 47(11):4037.
Application：Func, Human, MCF-7, MDA-MB-231 cells.
- Histone Deacetylase 3-Mediated Inhibition of microRNA-19a-3p Facilitates the Development of Rheumatoid Arthritis-Associated Interstitial Lung Disease.
Hui Yuan, Li Jiao, Nan Yu, Haifeng Duan, Yong Yu, Yanrong Bai.
Frontiers in Physiology 2020 Dec; 11:549656.
Application：Func, Human, HEK 293T cells.
- miR-135a deficiency inhibits the AR42J cells damage in cerulein-induced acute pancreatitis through targeting FAM129A.
Zhang KK, Yu SS, Li GY, He L, Liang XQ.
Pflügers Archiv 2019 Dec; 471(11-12):1519.
Application：Func, Human, HEK 293T cells.
- MiR-520b inhibited metastasis and proliferation of non-small cell lung cancer by targeting CHAF1A.
Cai Y, Dong ZY, Wang JY.
European Review for Medical and Pharmacological Sciences 2018 Nov; 22(22):7742.
Application：Func, Human, A-549 cells.
- Expression of AGPAT2, an enzyme involved in the glycerophospholipid/triacylglycerol biosynthesis pathway, is directly regulated by HIF-1 and promotes survival and etoposide resistance of cancer cells under hypoxia.
Triantafyllou EA, Georgatsou E, Mylonis I, Simos G, Paraskeva E.
Biochimica et Biophysica Acta 2018 Sep; 1863(9):1142.
Application：Func, Human, HEK 293 cells.
- Bilirubin-induced ER stress contributes to the inflammatory response and apoptosis in neuronal cells.
Qaisiya M, Brischetto C, Jašprová J, Vitek L, Tiribelli C, Bellarosa C.
Archives of Toxicology 2016 Aug; 91(4):1847.
Application：Quant, Human , SH-SY5Y cells.
- Interference of KLF9 relieved the development of gestational diabetes mellitus by upregulating DDAH2.