Histone H3 R8me2s antibody
Katalog-Nummer orb420395-50ug
Size : 50ug
Marke : Biorbyt
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Histone H3 R8me2s antibody
Catalog Number: orb420395
| Catalog Number | orb420395 |
|---|---|
| Category | Antibodies |
| Description | Histone H3 R8me2s antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ChIP, DOT, WB |
| Reactivity | C. elegans, Human |
| Isotype | IgG |
| Immunogen | Histone H3 [Sym-dimethyl Arg8] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic symmetric dimethylated peptide surrounding Arginine 8 of human Histone H3.2. |
| Concentration | 0.52mg/mL |
| Dilution range | ChIP: 2-5µg/million cells, WB: 2µg/mL |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Anti-Histone H3 [Sym-dimethyl Arg8] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
| Conjugation | Unconjugated |
| UniProt ID | Q71DI3 |
| NCBI | NP_001005464 |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-Histone H3 Sym-dimethyl Arg8 antibody, |
| Note | For research use only |
| Application notes | Anti-Histone H3 [Sym-dimethyl Arg8] antibody is tested for Western Blot, Chromatin Immunoprecipitation and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals. |
| Expiration Date | 12 months from date of receipt. |
Chromatin Immunoprecipitation of Histone H3 [Sym-dimethyl Arg8] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of Anti-Histone H3 R8 me2a was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input = 1).
Western Blot of Rabbit Anti-Histone H3 [Sym-dimethyl Arg8] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Sym-dimethyl Arg8] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [Sym-dimethyl Arg8] Antibody. Lane 1: R8Me2a. Lane 2: R8Me2s. Lane 3: R8Me1. Lane 4: Histone H3 R8. Load: 30 µg per lane. Primary antibody: Histone H3 [Sym-dimethyl Arg8] at 2 µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.