Human IFN-gamma ELISA Kit (96-well)
Specifications
Product Data | |
Format | 96-well strip plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Length | 3 hours |
Signal | Colorimetric |
Curve Range | 15.625-1000pg/ml |
Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
Sample Volume | 20 uL |
Specificity | Natural and recombinant Human IFN-? Ligand |
Sensitivity | 7pg/mL |
Reactivities | Human |
Interference | No significant interference observed with available related molecules. |
Components |
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Background | Interferon gamma (IFN-.) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA . Produced by Tlymphocytes and natural killer (NK) cells, IFN-. .is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-.. is reported to be active only on human and non-human primate cells . The biochemistry and biological activities of the interferons have been extensively reviewed . Human IFN-. .is a 143 amino acid residue, 20 or 25 kDa glycoprotein that demonstrates little sequence homology to IFN-.. or -... Naturally occurring IFN-. .is found as either of two molecular weight species, differing in degree of glycosylation. Human IFN-.. apparently exists as a head-to-tail dimer in solution with the C-terminus of one monomer aligned with the N-terminus of the other monomer A receptor for IFN-. has been identified and its gene localized to chromosome 6 Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-... Functionally, IFN-. .produces a variety of effects. Produced by CD8+, NK, gd, and TH1 T helper cells, IFN-. .has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes he antiprotozoal activity of IFN-. .against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-. ..The immunomodulatory effects of IFN-.. are extensive and diverse. In monocyte/macrophages, the activities of IFN-. .include: increasing the expression of class I and II MHC antigens; increasing the production of IL-1, platelet-activating factor, H2O2, and pterin; protection of monocytes against LAK cell-mediated lysis; downregulation of IL-8 mRNA expression that is upregulated by IL-2; and, with lipopolysaccharide, induction of NO production.Finally, IFN-..has been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells. |
Gene Symbol | IFNG |
Gene ID | 3458 |
Standard Curve | Representative standard curve for IFN-? ELISA. IFN-? was diluted in serial two-fold steps in Sample Diluent. |
Documents
SDS |