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IgE (Biotin)

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Katalog-Nummer I1900-01H-500ug

Size : 500ug

Marke : US Biological

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I1900-01H IgE (Biotin)

Clone Type
Polyclonal
Host
mouse
Source
rat
Swiss Prot
P01855
Isotype
IgG1
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:|Suitable for use in ELISA and Western Blot. Other applications have not been tested. ||Recommended Dilutions: |ELISA: 500ng/ml as detecting antibody|Optimal working dilutions to be determined by researcher.||Hybridoma: |Sp2/0 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2277 (MARE-1)|Host: mouse|Source: rat|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.|Purity: Purified by affinity chromatography from tissue culture supernatant.|Immunogen: Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.|Specificity: Recognizes the epsilon chain of rat IgE and does not crossreact with other classes of rat immunoglobulin. Binds to rat IgE with an avidity of 4x10e9M-1||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.
Purity
Purified by affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the epsilon chain of rat IgE and does not crossreact with other classes of rat immunoglobulin. Binds to rat IgE with an avidity of 4x10e9M-1
References
1. Bazin, H., et al., J. Immunol. 121: 2077-2081 (1987). 2. Bazin, H., et al., J. Imm. Methods 71: 9-16 (1984). 3. Bazin, H., et al., J. Imm. Methods 66: 261-269 (1984). 4. Negrao-Cooea, D., et al., J. Immunol. 157: 4037-4044 (1996). 5. Bazin, H., et al., Immunology 26: 713-723 (1974).