IgG, H&L (Biotin)

Applications: |Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|ELISA: 1:20,000-1:160,000. 1ug of donkey IgG was used in a standard capture ELISA with ABTS (A0030) as a substrate for 30 minutes at RT.|Western Blot: 1:2000-1:10,000|Immunohistochemistry: 1:1000-1:5000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.