LCLC-97TM1
Marke : CLS Cell Lines Service
LCLC-97TM1 Cells
General information
Description | In vitro etablished fro the primary lung large cell carcinoma |
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Organism | Human |
Tissue | Lung |
Disease | Large cell carcinoma |
Synonyms | LCLC97TM1 |
Characteristics
Age | 44 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | LCLC-97TM1 (Cytion catalog number 300409) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 expression |
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Tumorigenic | Yes, in nude mice |
Reverse transcriptase | negative |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:6 is recommended |
Seeding density | 1 to 3 x 10^5 cells/cm^2 |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | CSF1PO: 10,11 D13S317: 11,13 D16S539: 12,13 D5S818: 10,12 D7S820: 10,11 TH01: 8 TPOX: 8,11 vWA: 19,20 D3S1358: 15 D21S11: 27,30 D18S51: 16 Penta E: 15 Penta D: 12,15 D8S1179: 14 FGA: 23 |
HLA alleles | A*: 02:01:01, 24:02:01 B*: 15:01:01, 18:01:01 C*: 03:03:01, 12:03:01 DRB1*: 01:01:01, 04:01:01 DQA1*: 01:01:01, 03:01:01 DQB1*: 03:02:01, 05:01:01 DPB1*: 04:02:01 E: 01:03:02 |