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LTF Antibody

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Katalog-Nummer AP8698A

Size : 400ul

Marke : Abcepta

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  • WB - LTF Antibody AP8698A
    Western blot analysis of LTF Antibody (Cat. #AP8698a) in MDA-MB231 cell line lysates (35ug/lane). LTF (arrow) was detected using the purified Pab.
  • WB - LTF Antibody AP8698A
    Western blot analysis of LTF Antibody (Cat. #AP8698a) in mouse spleen tissue lysates (35ug/lane). LTF (arrow) was detected using the purified Pab.
  • WB - LTF Antibody AP8698A
    All lanes : Anti-LTF Antibody at 1:1000-2000 dilution Lane 1: Human plasma tissue lysate Lane 2: Hela whole cell lysate Lane 3: Human uterus tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - LTF Antibody AP8698A
    All lanes : Anti-LTF Antibody at 1:1000 dilution Lane 1: Human plasma tissue lysate Lane 2: Human fetal kidney tissue lysate Lane 3: Human uterus tissue lysate Lane 4: Human skeletal muscle tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - LTF Antibody AP8698A
    All lanes : Anti-LTF Antibody at 1:4000 dilution Lane 1: A549 whole cell lysate Lane 2: Human plasma tissue lysate Lane 3: Human fetal kidney tissue lysate Lane 4: Human breast tissue lysate Lane 5: Human uterus tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • FC - LTF Antibody AP8698A
    LTF Antibody (Cat. #AP8698a) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P02788
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 78182 Da
Additional Information
Gene ID 4057
Other Names Lactotransferrin, Lactoferrin, 3421-, Growth-inhibiting protein 12, Talalactoferrin, Lactoferricin-H, Lfcin-H, Kaliocin-1, Lactoferroxin-A, Lactoferroxin-B, Lactoferroxin-C, LTF, GIG12, LF
Target/Specificity This LTF antibody is generated from rabbits immunized with LTF recombinant protein.
Dilution WB~~1:4000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLTF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LTF (HGNC:6720)
Synonyms GIG12, LF
Function Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Cellular Location [Isoform 1]: Secreted. Cytoplasmic granule. Note=Secreted into most exocrine fluids by various endothelial cells Stored in the secondary granules of neutrophils
Tissue Location High levels are found in saliva and tears, intermediate levels in serum and plasma, and low levels in urine. In kidney, detected in the distal collecting tubules in the medulla but not in the cortical region or in blood vessels. Detected in peripheral blood neutrophils (at protein level). Isoform 1 and isoform DeltaLf are expressed in breast, prostate, spleen, pancreas, kidney, small intestine, lung, skeletal muscle, uterus, thymus and fetal liver Isoform 1 is expressed in brain, testis and peripheral blood leukocytes; isoform DeltaLf is barely detectable in these tissues Isoform DeltaLf is expressed in placenta, liver and ovary; isoform 1 is barely detectable in these tissues. In kidney, isoform 1 is expressed at high levels in the collecting tubules of the medulla but at very low levels in the cortex.