Panc-1
Marke : CLS Cell Lines Service
Panc-1 Cells
Essential facts about the PANC-1 cell line
Description | PANC-1 cells, originating from a pancreatic duct carcinoma in a 56-year-old Caucasian male, stand as a pivotal epithelial cell line in the realm of cancer research, particularly in the study of pancreatic carcinoma. Panc1 cells offer a useful model for delving into the intricacies of pancreatic cancer, including ductal adenocarcinoma cell lines and their tumorigenic potential. The cells' epithelial morphology and their capacity to exhibit diverse morphological patterns underscore their relevance in mimicking the clonal heterogeneity and complex tumor microenvironment seen in pancreatic ductal adenocarcinoma (PDAC). PANC-1 cells express markers such as vimentin and somatostatin receptors like SSTR2, which play a crucial role in neuroendocrine differentiation. This expression profile, coupled with the cells' ability to undergo epithelial-mesenchymal transition (EMT) marker expression and EMT subtype shifting, makes them an excellent platform for exploring therapeutic strategies targeting the EMT process and neuroendocrine features of pancreatic cancer. The cell line's karyotypic analysis reveals a hyperdiploid state with notable genetic alterations, including the loss of the Y chromosome and mutations in critical genes such as CDKN2A and the p53 gene. In summary, PANC-1 cells provide a multifaceted model for pancreatic cancer research, enabling detailed investigations into the phenotype and genotype of pancreatic adenocarcinoma, the efficacy of targeted therapies, and the molecular mechanisms driving cancer progression. |
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Organism | Human |
Tissue | Pancreas |
Disease | Adenocarcinoma |
Synonyms | PANC-1, PANC.1, Panc 1, PanC1, Panc1, PANC1, Panc-1-P |
Details
Age | 56 years |
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Gender | Male |
Ethnicity | Caucasian |
Growth properties | Adherent |
Documentation about the pancreatic cancer cell line Panc-1
Citation | Panc-1 (Cytion catalog number 300228) |
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Biosafety level | 1 |
Genotype
Protein expression | p53 positive, CEA negative |
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Isoenzymes | G6PD, B |
Tumorigenic | Growth in soft agar. Formation of progressively growing carcinomas in nude athymic mice. |
Mutational profile | Panc-1 cells carry a heterozygous Kras mutation in codon12: GGT(Wt Gly) >GAT(Asp) |
Karyotype | Three distinct marker chromosomes and one 1 ring chromosome |
Cell culture practices for the pancreas cell line Panc1
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 10,12 D13S317: 11 D16S539: 11 D5S818: 11,13 D7S820: 8,10 TH01: 7,8 TPOX: 8,11 vWA: 15 D3S1358: 17 D21S11: 28 D18S51: 12 D8S1179: 14,15 FGA: 21 D1S1656: 12,14 D2S1338: 23,24 D12S391: 22 D19S433: 11,16 |
HLA alleles | A*: 02:01:01, 11:01:01 B*: 01.01.1900 14:01 C*: 12:03:01 DRB1*: 13:01:01 DQA1*: 01:03:01 DQB1*: 06:03:01 DPB1*: 02:01:02G, 04:02:01G E: 01:01, 01:03 |