PDE3A TR-FRET BioAssay™ Kit

Katalog-Nummer 171964-1Kit

Size : 1Kit

Marke : US Biological

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171964 PDE3A TR-FRET BioAssay™ Kit

Clone Type
Polyclonal
Shipping Temp
Blue Ice
Storage Temp
-20°C/-70°C

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE3A, also known as cGMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility. ||The PDE3A TR-FRET BioAssay™ Kit comes in a convenient 96-well format, with purified PDE3A enzyme, fluorescently labeled PDE substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. Using this kit, only two simple steps on a microtiter plate are required for the PDE3A activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE3A for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 1 hour. Then, fluorescence intensity can be measured using a fluorescence reader. The PDE3A TR-FRET Assay Kit is designed for identification of inhibitors of PDE3A using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology. The assay is based on the generation of FAM-labeled nucleotide monophosphates by the phosphodiesterase. These phosphate groups bind to terbium-labeled nanoparticles, resulting in energy transfer from the terbium to the FAM, which emits a fluorescent signal at 520 nm. The change in fluorescent intensity can be easily measured using a fluorescence plate reader. ||Applications:|Great for screening small molecular inhibitors for drug discovery and HTS applications.||Components:|PDE3A recombinant enzyme, 1x1ug|FAM-Cyclic-3’, 5’-AMP: 20um, 1x50ul|PDE Assay Buffer, 1x25ml|Tb donor, 1x30ml|Binding Agent, 1x200ul|Binding Buffer A, 1x20ml|Binding Buffer B, 1x20ml|Black, low binding NUNC microtiter plate, 1 plate||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Maurice, DH. Front. Biosci. 2005; 10:1221-8.