Pronase reagent (Ready-to-use)
Katalog-Nummer GTX73181
Size : 100ml
Marke : Genetex
Contact local distributor :
Telefonnummer : +1 850 650 7790
Application | IHC |
|---|---|
| Package | 100 ml |
Datasheet File View product citations for antibody GTX73181 on CiteAb
PRODUCT
Summary
Pronase Reagent
APPLICATION
Application Note
For IHC: Use as antigen retriever for some antibodies in IHC. Reagent is supplied in ready-to-use format. Over-digestion may detach tissue sections from slides or may result in the loss of tissue morphology. It may be necessary to use + charge, sialanized or polylysine coated slides.
PROPERTIES
Form
Liquid
Storage
Keep as concentrated solution, aliquot and store at 4ºC. DO NOT FREEZE.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Background
Pronase is used for photolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Application Reference
Sun JD et al. Clin Cancer Res 2012; 18 (3) : 758-70 Selective tumor hypoxia targeting by hypoxia-activated prodrug TH-302 inhibits tumor growth in preclinical models of cancer.
REVIEW
Application Reference
Sun JD et al. Clin Cancer Res 2012; 18 (3):758-70 Selective tumor hypoxia targeting by hypoxia-activated prodrug TH-302 inhibits tumor growth in preclinical models of cancer.
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