SF126
Marke : CLS Cell Lines Service
SF126 Cells
General information
Organism | Human |
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Tissue | Brain, left frontal lobe |
Disease | Glioblastoma |
Applications | cell biology studies of gliomas |
Synonyms | SF-126, SF 126 |
Characteristics
Age | 50 years |
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Gender | Female |
Ethnicity | European |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SF126 (Cytion catalog number 300608) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | No (tested in athymic mice) |
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Products | procollagen III, forms collagen fibers in vitro (interstitial collagen synthesis) |
Ploidy status | Aneuploid |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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