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> Thiazole Orange [107091-89-4]

Thiazole Orange [107091-89-4]

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Katalog-Nummer HY-D0150-100mg

Size : 100mg

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Thiazole Orange is an asymmetric anthocyanin dye that can be coupled with oligonucleotides (ONs) to prepare fluorescent hybridization probes. Thiazole Orange can be used for reticulocyte analysis. λEx/λEm (including DNA) of Thiazole Orange =510/527 nm.

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Thiazole Orange Chemische Struktur

Thiazole Orange Chemische Struktur

CAS. Nr. : 107091-89-4

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Beschreibung

Thiazole Orange is an asymmetric anthocyanin dye that can be coupled with oligonucleotides (ONs) to prepare fluorescent hybridization probes. Thiazole Orange can be used for reticulocyte analysis. λEx/λEm (including DNA) of Thiazole Orange =510/527 nm[1][2][3].

Molekulargewicht

476.61

Formel

C26H24N2O3S2
CAS. Nr.

107091-89-4
Appearance

Solid

Color

Pink to red

SMILES

CN1/C(SC2=CC=CC=C12)=C/C3=C4C=CC=CC4=[N+](C)C=C3.O=S(C5=CC=C(C)C=C5)([O-])=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 25 mg/mL (52.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

MEOH : 25 mg/mL (52.45 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0982 mL 10.4908 mL 20.9815 mL
5 mM 0.4196 mL 2.0982 mL 4.1963 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (2.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation

Purity: 99.78%

COA (255 KB) LCMS (278 KB)

Handling Instructions (2659 KB)
Verweise

  • [1]. Linda G. Le, et al. Thiazole Orange: A New Dye for Reticulocyte Analysis. cytometry 7:508-517 (1986)

    [2]. Piotr Klimkowski, et al. Design of thiazole orange oligonucleotide probes for detection of DNA and RNA by fluorescence and duplex melting. Org Biomol Chem. 2019 Jun 28; 17(24): 5943–5950.  [Content Brief]

    [3]. O'Neil CS, et al. DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes. J Vis Exp. 2019 Mar 31;(145)  [Content Brief]

  • [1]. Linda G. Le, et al. Thiazole Orange: A New Dye for Reticulocyte Analysis. cytometry 7:508-517 (1986)

    [2]. Piotr Klimkowski, et al. Design of thiazole orange oligonucleotide probes for detection of DNA and RNA by fluorescence and duplex melting. Org Biomol Chem. 2019 Jun 28; 17(24): 5943–5950.

    [3]. O'Neil CS, et al. DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes. J Vis Exp. 2019 Mar 31;(145)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / MEOH 1 mM 2.0982 mL 10.4908 mL 20.9815 mL 52.4538 mL
5 mM 0.4196 mL 2.0982 mL 4.1963 mL 10.4908 mL
10 mM 0.2098 mL 1.0491 mL 2.0982 mL 5.2454 mL
15 mM 0.1399 mL 0.6994 mL 1.3988 mL 3.4969 mL
20 mM 0.1049 mL 0.5245 mL 1.0491 mL 2.6227 mL
25 mM 0.0839 mL 0.4196 mL 0.8393 mL 2.0982 mL
30 mM 0.0699 mL 0.3497 mL 0.6994 mL 1.7485 mL
40 mM 0.0525 mL 0.2623 mL 0.5245 mL 1.3113 mL
50 mM 0.0420 mL 0.2098 mL 0.4196 mL 1.0491 mL
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Thiazole Orange Related Classifications

  • Others
  • Fluorescent Dye
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thiazole Orange107091-89-4Fluorescent DyeReticulocytesNucleicAcidDyesOligonucleotidesConjugatedInhibitorinhibitorinhibit

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