Topoisomerase II alpha (TOP2A) (C-term) Mouse Monoclonal Antibody [Clone ID: 1C5]
CAT#: AM26595AF-N
Topoisomerase II alpha (TOP2A) (C-term) mouse monoclonal antibody, clone 1C5, Azide Free
Specifications
Product Data | |
Clone Name | 1C5 |
Applications | FC, IF, IHC, IP, WB |
Recommended Dilution | Western blot: 0.1-0.5 µg/ml for chemiluminescence detection system. Immunoprecipitation: 1-10 µg/200-300 µg/ml of cell extract. Immunohoistochemistry on paraffin sections: Heat treatment is necessary; Microwave oven; 2 times for 10 minutes aech in 10 mM citrate buffer (pH 6.5). Immunocytochemistry: 1-10 µg/ml. Flow cytometry: 0.1-1 µg/ml (final contentration). |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Recombinant human Topoisomerase II alpha protein corresponding to C-termional 182 aa |
Specificity | This antibody detects 170 kDa of human Topo II alpha on Western blot with total cell lysate from human cell lines. |
Formulation | PBS containing 50 % glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | topoisomerase (DNA) II alpha |
Database Link | |
Background | Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase II alpha is also implicated in drug resistance of tumor cells. |
Synonyms | TOP2, Top-2, DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme |
Note | This product was originally produced by MBL International. |
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