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> UZH2 [2756566-45-5]

UZH2 [2756566-45-5]

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Katalog-Nummer HY-115717-10mg

Size : 10mg

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UZH2 is a potent and selective METTL3 inhibitor with an IC50 value of 5 nM.

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UZH2 Chemische Struktur

UZH2 Chemische Struktur

CAS. Nr. : 2756566-45-5

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Beschreibung

UZH2 is a potent and selective METTL3 inhibitor with an IC50 value of 5 nM.

In Vitro

UZH2 (compound 22) (0-25 μM, 1 h) stabilizes METTL3 in a dosedependent manner at 54 °C， with an EC50 of 0.85 μM in MOLM-13 cells[1].
UZH2 (16 h) reduces m6A/A ratio in polyadenylated RNA in MOLM-13 and PC-3 cells，with the EC50 of 0.7 and 2.5 μM, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

UZH2 Related Antibodies

Western Blot Analysis[1]

Cell Line: MOLM-13 cells
Concentration: 0-25 μM
Incubation Time: 1h
Result: Stabilized METTL3 in a dosedependent manner at 54 °C.
Molekulargewicht

513.63

Formel

C27H37F2N7O
CAS. Nr.

2756566-45-5
Appearance

Solid

Color

White to off-white

SMILES

CNC1=NC=NC(N2CCC3(CN(CC(N3)=O)C4=C(C=C(C(F)=C4)CN5CCC(C)(CC5)C)F)CC2)=C1
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 50 mg/mL (97.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9469 mL 9.7346 mL 19.4693 mL
5 mM 0.3894 mL 1.9469 mL 3.8939 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (2.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
Verweise

  • [1]. Dolbois A, et al. 1,4,9-Triazaspiro[5.5]undecan-2-one Derivatives as Potent and Selective METTL3 Inhibitors. J Med Chem. 2021 Sep 9;64(17):12738-12760.  [Content Brief]

  • [1]. Dolbois A, et al. 1,4,9-Triazaspiro[5.5]undecan-2-one Derivatives as Potent and Selective METTL3 Inhibitors. J Med Chem. 2021 Sep 9;64(17):12738-12760.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9469 mL 9.7346 mL 19.4693 mL 48.6732 mL
5 mM 0.3894 mL 1.9469 mL 3.8939 mL 9.7346 mL
10 mM 0.1947 mL 0.9735 mL 1.9469 mL 4.8673 mL
15 mM 0.1298 mL 0.6490 mL 1.2980 mL 3.2449 mL
20 mM 0.0973 mL 0.4867 mL 0.9735 mL 2.4337 mL
25 mM 0.0779 mL 0.3894 mL 0.7788 mL 1.9469 mL
30 mM 0.0649 mL 0.3245 mL 0.6490 mL 1.6224 mL
40 mM 0.0487 mL 0.2434 mL 0.4867 mL 1.2168 mL
50 mM 0.0389 mL 0.1947 mL 0.3894 mL 0.9735 mL
60 mM 0.0324 mL 0.1622 mL 0.3245 mL 0.8112 mL
80 mM 0.0243 mL 0.1217 mL 0.2434 mL 0.6084 mL
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UZH2 Related Classifications

Help & FAQs

Keywords:

UZH22756566-45-5UZH 2UZH-2METTL3Methyltransferase-like 3Inhibitorinhibitorinhibit

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