Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity), a luminol-based chemiluminescent substrate is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP)-conjugated secondary antibodies. It is designed for the detection of the target proteins in amounts that are too small to be seen with typical ECL substrates.
| Target | Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity) |
| Tested Applications | WB |
| Storage | Store at room temperature for up to 24 months. Avoid exposure to light. |
| Buffer | Not applicable. |
| Availability | Shipped within 10-15 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
| Directions for use | - Keep the membrane moist in the wash buffer while preparing the substrate mixture. Please ensure the membrane does not dry out during the subsequent steps.
- Mix Luminol solution and Peroxide Solution in a 1:1 ratio, and thoroughly mix the chemiluminescent substrate solution well.
- Prepare 0.1 ml of solution per cm2 of membrane. For a mini-sized membrane (7 × 8.5 cm), 5 ml of solution is sufficient. For a midi-sized membrane (8.5 × 13.5 cm), 10 ml of solution is sufficient.
- Place the membrane with the protein side up on a clear and level surface or in a clean container.
- Remove the membrane from the chemiluminescent substrate solution and drain off excessive solution.
- Place the membrane in a plastic sheet protector or in plastic wrap to prevent the membrane from drying.
- Image the membrane with a digital imager or by exposure to X-ray film.
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Research Articles on Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity) 
