Construction of basic vectors
Vectors built from standard backbones and standard components
Turnaround time for basic vectors:
Vector type
Turnaround*
shRNA vector
14-21 days
gRNA vector (single RNA)
14-21 days
gRNA vector (dual RNA)
21-27 days
Expression vector
16-22 days
* Turnaround time is defined as the time from when an order is accepted to when it is shipped. It does not include waiting time for any materials that the customer needs to supply (e.g. template DNA). Adenoviral vectors may have longer turnaround time.
Deliverables
By default, we ship vectors in the format of E. coli stock. Upon request, small amount of plasmid DNA can be provided if available. Two scales of high-quality plasmid DNA can be purchased when adding vector construction service into shopping cart: Miniprep (> 10 ug) and Maxiprep (> 300 ug, endotoxinfree, sterile).
Quality control
All constructed vectors have 100%sequence guarantee. Our vectors undergo stringent quality control to ensure that they contain the correct sequence exactly as designed. Common QC includes DNA quantification, A260/280 measurement, restriction enzyme digestion, Sanger sequencing, recovering bacteria from glycerol stock, and re-transformation.
Turnaround time
We offer top-quality vector construction services at unbeatable price with rapid turnaround time. Outsourcing cloning to us is often cheaper than the cost of reagents you will need to do it yourself, let alone savings on your time, effort and headaches.