A4548-1mg | α-Amanitin [23109-05-9] Clinisciences

Background

α-Amanitin, the most deleterious toxin of A. phalloides to humans, inhibits RNA polymerase II (RNAPII), causing hepatic and renal failure.

1. Duo Wu, Huang Huang, et al. "The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination." Cell Discov. 2023 Oct 3;9(1):99. PMID: 37789001
2. Bei Wang, Arabella H Wan, et al. "Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity." Nat Commun. 2023 May 16;14(1):2241. PMID: 37193694
3. Jiangli Lang, Chen Yang, et al. "High Glucose Activates ERK1/2 to Stabilize AP1 and Increase MMP9 Expression in Diabetic Foot Ulcers." Exp Cell Res. 2021 Mar 3;112550. PMID:33675806
4. Huo X, Ji L, et al. "The Nuclear Matrix Protein SAFB Cooperates with Major Satellite RNAs to Stabilize Heterochromatin Architecture Partially through Phase Separation." Mol Cell. 2019 Oct 28. pii: S1097-2765(19)30761-0. PMID:31677973

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	918.97
Cas No.	23109-05-9
Formula	C₃₉H₅₄N₁₀O₁₄S
Solubility	≥1 mg/mL in H2O; Soluble in Ethanol
SDF	Download SDF
Canonical SMILES	CCC(C)C1C(=O)NCC(=O)NC2CS(=O)C3=C(CC(C(=O)NCC(=O)N1)NC(=O)C(NC(=O)C4CC(CN4C(=O)C(NC2=O)CC(=O)N)O)C(C)C(CO)O)C5=C(N3)C=C(C=C5)O
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Cell experiment:[1]
Cell lines	Mouse blastocysts
Reaction Conditions	1.1 μg/ml α-amanitin
Applications	α-Amanitin inhibited the incorporation of ³H-UMP into embryos. α-Amanitin at the concentration of 1.1 μg/ml was found to inhibit RNA polymerase activity by 32%.
Animal experiment:[1]
Animal models	Preimplantation mouse embryos at the two-cell stage of development collected from CF1 female mice
Dosage form	0 ~ 1 μg/ml α-amanitin 48 h
Applications	Less than 1% of the embryos which had been treated with 1 μg/ml of α-amanitin developed to the morula or blastocyst stages. α-Amanitin can be used to determine which forms of RNA polymerase are active and the relative amounts of each form which are present in preimplantation mouse embryos.
Note	The technical data provided above is for reference only.
	References: 1. Warner CM, Versteegh LR. In vivo and in vitro effect of alpha-amanitin on preimplantation mouse embryo RNA polymerase. Nature, 1974, 248(5450): 678-680.

Purity ≥90%