14-3-3, alpha, beta (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor protein-1) (APC)

14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 expression is particularly high in certain neural tissues and studies in Drosophila have established a role for 14-3-3 proteins in nervous system development and function. Recently, a dimeric 14-3-3 protein was determined to be an essential cofactor for Raf kinase activity.||Applications: |Suitable for use in FLISA and Western Blot. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.