2.5% Trypsin-EDTA Solution 10X
Cat# sc-391060A
Size : 1L
Brand : Santa Cruz Biotechnology
2.5% Trypsin-EDTA Solution 10X (CAS 9002-07-7 (solid))
Noms alternatifs:
2.5% Trypsin-EDTA Solution 10X is also known as Tryptase.
Application:
2.5% Trypsin-EDTA Solution 10X is an agent used to disengage adherent cells from tissue culture plates.
Numéro CAS:
9002-07-7 (solid)
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
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SDS & Certificat d’Analyses
The 2.5% Trypsin-EDTA Solution 10X is a concentrated enzymatic preparation commonly used in cell culture research to dissociate adherent cells from the substrate or each other, facilitating their harvest and passage. Trypsin, a serine protease derived from porcine pancreas, cleaves peptide bonds in proteins, targeting primarily those on the carboxyl side of lysine and arginine residues. This action helps to break down the protein structures that hold cells together or attach them to the culture dish surface. EDTA, or ethylenediaminetetraacetic acid, is included in this solution to enhance the effectiveness of trypsin. EDTA acts as a chelating agent, binding divalent cations such as calcium and magnesium that are critical for cell adhesion. By removing these ions, EDTA reduces the strength of cell-to-cell and cell-to-substrate adhesion, thus synergistically working with trypsin to facilitate cell detachment without excessive damage. In scientific research, this trypsin-EDTA solution is crucial for cell culture applications, particularly when researchers need to maintain cell viability and functionality post-harvest. The enzymatic action allows for the gentle separation of cells, preserving important surface receptors and cell integrity, which is essential for downstream applications like flow cytometry, cell sorting, and tissue engineering studies. The 10X concentration provides a convenient stock solution that can be diluted to the desired strength based on specific experimental needs, offering flexibility and efficiency in experimental workflows.
References:
- [Long-term in vitro culture of the fibroblasts from the deep partial thickness burn wound in burn patients]. | Li, YL., et al. 2003. Zhonghua Shao Shang Za Zhi. 19: 35-7. PMID: 12678974
- Increased proteolysis of collagen in an in vitro tensile overload tendon model. | Willett, TL., et al. 2007. Ann Biomed Eng. 35: 1961-72. PMID: 17763961
- Different methods of detaching adherent cells significantly affect the detection of TRAIL receptors. | Zhang, B., et al. 2012. Tumori. 98: 800-3. PMID: 23389369
- Biophysical characterization of low-frequency ultrasound interaction with dental pulp stem cells. | Ghorayeb, SR., et al. 2013. J Ther Ultrasound. 1: 12. PMID: 25516801
- Atomic force microscopy in the production of a biovital skin graft based on human acellular dermal matrix produced in-house and in vitro cultured human fibroblasts. | Łabuś, W., et al. 2018. J Biomed Mater Res B Appl Biomater. 106: 726-733. PMID: 28323389
- A novel autophagy modulator 6-Bio ameliorates SNCA/ alpha -synuclein toxicity. | Suresh, SN., et al. 2017. Autophagy. 13: 1221-1234. PMID: 28350199
- Development of a new method for the preparation of an acellular allodermis, quality control and cytotoxicity testing. | Dragúová, J., et al. 2017. Cell Tissue Bank. 18: 153-166. PMID: 28405854
- Specific capture, recovery and culture of cancer cells using oriented antibody-modified polystyrene chips coated with agarose film. | Jeong, J., et al. 2018. Colloids Surf B Biointerfaces. 162: 306-315. PMID: 29220830
- A simple trypsin resistance assay for muscle and other cell fusion. | Denning, G. and Fulton, AB. 1986. J Histochem Cytochem. 34: 959-62. PMID: 3086428
- Collagen Fibrils Mechanically Contribute to Tissue Contraction in an In Vitro Wound Healing Scenario. | Brauer, E., et al. 2019. Adv Sci (Weinh). 6: 1801780. PMID: 31065517
- Estimation and in-situ detection of thorium in human liver cell culture by arsenazo-III based colorimetric assay. | Yadav, R., et al. 2020. Biometals. 33: 75-85. PMID: 31897857
- Establishing Sustainable Cell Lines of a Coral, Acropora tenuis. | Kawamura, K., et al. 2021. Mar Biotechnol (NY). 23: 373-388. PMID: 33899125
- Morphologic changes in rat urothelial cells during carcinogenesis: I. Histologic and cytologic changes. | King, EB., et al. 1984. Cytometry. 5: 447-53. PMID: 6489059
- Primary culture of bovine gall bladder epithelial cells. | Plevris, JN., et al. 1993. Gut. 34: 1612-5. PMID: 8244152
Formulation :
Solution in HBSS. 2.5% trypsin, 0.2% EDTA solution in saline, lacking phenol red
État Physique :
Liquid
STOCKAGE :
Store at -20° C
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
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2.5% Trypsin-EDTA Solution 10X | CAS 9002-07-7 (solid) SAMPLE Certificate of AnalysisCERTIFICAT D'ANALYSE
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Catalog Number:sc-391060
Lot Number:SAMPLE
Product Name:2.5% Trypsin-EDTA Solution 10X
Test | Specification | Results |
---|---|---|
Appearance | Clear liquid | |
pH | 7.5 | |
Sterility | Filtered with 0.22 µm filter |
Informations pour la commande
Nom du produit | Ref. Catalogue | COND. | Prix HT | QTÉ | Favoris | |
2.5% Trypsin-EDTA Solution 10X, 100 ml | sc-391060 | 100 ml | .00 | |||
2.5% Trypsin-EDTA Solution 10X, 250 ml | sc-391060B | 250 ml | .00 | |||
2.5% Trypsin-EDTA Solution 10X, 1 L | sc-391060A | 1 L | .00 |