Anti-GGA3 (RABBIT) Antibody
Cat# 600-401-893
Size : 100ug
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for GGA3 Antibody
Product Details
Anti-GGA3 (RABBIT) Antibody - 600-401-893
rabbit anti-GGA3 antibody, GGA-3, GGA 3, ADP-ribosylation factor-binding protein GGA3, Golgi-localized gamma ear-containing ARF-binding protein 3
Rabbit
Polyclonal
IgG
Target Details
GGA3 - View All GGA3 Products
Human, Chimpanzee
Conjugated Peptide
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 400-425 of Human GGA3.
This affinity purified antibody is directed against human GGA3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity may occur with GGA3 from macaque (93% homology) and orangutan (87% homology) sources. The immunizing sequence is present on both the long (isoform 1) and short (isoform 2) transcript variants for this protein.
Q9NZ52 - UniProtKB
20336267 - NCBI Protein
23163 - Gene ID
Application Details
ELISA, WB
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at 78 kDa in size corresponding to GGA3 by western blotting in the appropriate cell lysate or extract.
Formulation
1.0mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
GGA3 (also known as ADP-ribosylation factor binding protein GGA3, Golgi-localized, gamma ear-containing, ARF-binding protein 3, Gamma-adaptin related protein 3) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200, GGA1 and GGA2 as well as RABEP1, RABGEF1 and type-I membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. GGA3 is a ubiquitously expressed membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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