Sandwich ELISA using RM123 as the capture antibody ELISA of human immunoglobulins shows RM123 A titer ELISA using RM123. The plate was coated
Product Details Product Name Anti-Human IgD Rabbit Monoclonal Antibody, Clone RM123 Product Description Rabbit monoclonal to Human IgD Catalog No. 31-1025-00 Clone Name RM123 Product Data Sheet Anti-Human IgD Rabbit Monoclonal Antibody Data Sheet RM123 Specificity RM123 reacts to human IgD. No cross reactivity with human IgG, IgM, IgE, or IgA. Immunogen Human IgD Application ELISA Species Reactivity Human Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 25 ng/well – 200 ng/well (for Capture);
0.01 µg/mL – 0.1 µg/mL (for Detection); Keywords . Product Images
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
reacts to IgD from human plasma and IgDκ from
human myeloma. No cross reactivity with human
IgG, IgM, IgA, or IgE. The plate was coated with
50 ng/well of different immunoglobulins. 200 ng/mL,
50 ng/mL, or 10 ng/mL of RM123 was used as the
primary antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
with different amounts of human IgD (from plasma).
A serial dilution of RM123 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.