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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-IDE antibody (Clone: 3H4)

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Cat# NB-22-6983-50

Size : 50ug

Contact local distributor :

Phone : +1 850 650 7790

Brand : Neo Biotech

Contact local distributor :

Phone : +1 850 650 7790

Immunofluorescence analysis of Human-breast tissue. 1, IDE monoclonal antibody (3H4) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) HepG2, diluted at 1:2000
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, IDE monoclonal antibody (3H4) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

General Info

Host:	Mouse
Applications:	WB/IHC/IF
Reactivity:	Human/Hamster
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Insulin-degrading enzyme is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	3H4
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range:	WB 1:1000 IF 1:200 IHC 1:50-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	IDE
Gene ID:	3416
Uniprot ID:	IDE_HUMAN
Specificity:	The antibody detects endogenous IDE proteins.
Immunogen:	Synthetic Peptide of IDE

Description

Post Translational Modifications	The N-terminus is blocked.
Function	Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin. Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP. Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP. Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients. Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).
Protein Name	Insulin-Degrading Enzyme Abeta-Degrading Protease Insulin Protease Insulinase Insulysin
Database Links	Reactome: R-HSA-5689880 Reactome: R-HSA-77387 Reactome: R-HSA-9033241
Cellular Localisation	Cytoplasm Cytosol Cell Membrane Secreted Present At The Cell Surface Of Neuron Cells The Membrane-Associated Isoform Is Approximately 5 Kda Larger Than The Known Cytosolic Isoform
Alternative Antibody Names	Anti-Insulin-Degrading Enzyme antibody Anti-Abeta-Degrading Protease antibody Anti-Insulin Protease antibody Anti-Insulinase antibody Anti-Insulysin antibody Anti-IDE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance