Reagents and instruments for immunology, cell biology and molecular biology.
 
> Anti-IDH1 antibody (1-414)

Anti-IDH1 antibody (1-414)

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Cat# NB-22-36098-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

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Phone : +1 850 650 7790

  • Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g IDH1 antibody . Western blot was performed from the immunoprecipitate using IDH1 antibody at a dilution of 1:1000.
  • Immunofluorescence analysis of L929 cells using [KO Validated] IDH1 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using [KO Validated] IDH1 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and IDH1 knockout (KO) 293T cells, using [KO Validated] IDH1 Rabbit polyclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

General Info

Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IDH1 (1-414) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: IDH1
Gene ID: 3417
Uniprot ID: IDHC_HUMAN
Immunogen Region: 1-414
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-414 of human IDH1 (NP_005887.2).
Immunogen Sequence: MSKKISGGSVVEMQGDEMTR IIWELIKEKLIFPYVELDLH SYDLGIENRDATNDQVTKDA AEAIKKHNVGVKCATITPDE KRVEEFKLKQMWKSPNGTIR NILGGTVFREAIICKNIPRL VSGWVKPIIIGRHAYGDQYR ATDFVVPGPGKVEITYTPSD GTQKVTYLVHNFEEGGGVAM GMYNQDKSIEDFAHSSFQMA LSKGWPLYLSTKNTILKKYD GRFKDIFQEIYDKQYKSQF

Description

Post Translational Modifications Acetylation at Lys-374 dramatically reduces catalytic activity.
Function Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency.
Protein Name Isocitrate Dehydrogenase Nadp Cytoplasmic
Idh
Idh1
Cytosolic Nadp-Isocitrate Dehydrogenase
Idpc
Nadp(+-Specific Icdh
Oxalosuccinate Decarboxylase
Database Links Reactome: R-HSA-2978092
Reactome: R-HSA-389542
Reactome: R-HSA-6798695
Reactome: R-HSA-9033241
Reactome: R-HSA-9818025
Cellular Localisation Cytoplasm
Cytosol
Peroxisome
Alternative Antibody Names Anti-Isocitrate Dehydrogenase Nadp Cytoplasmic antibody
Anti-Idh antibody
Anti-Idh1 antibody
Anti-Cytosolic Nadp-Isocitrate Dehydrogenase antibody
Anti-Idpc antibody
Anti-Nadp(+-Specific Icdh antibody
Anti-Oxalosuccinate Decarboxylase antibody
Anti-IDH1 antibody
Anti-PICD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance