Goat Polyclonal

Application / Dilution
FC1:1000
ICC1:200
IHC1:1000
WB1:2000

Size 0.5 ml

Species Reactivity Ms

DyLight® fluorophores have absorption maxima covering the entire visible light spectrum (350 nm to 777 nm), as well several key near-infrared and infrared wavelengths. Both the absorption and emission properties of the DyLight® fluorophores match the excitation and detection wavelengths of common fluorescence instrumentation used for immunocytochemistry, immunohistochemistry, and flow cytometry. The DyLight® fluorophores exhibit higher fluorescence intensity and photostability than Alexa Fluor, CyDye and LI-COR Dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9). Additionally, the water solubility of the DyLight® fluorophores allows a high dye-to-protein ratio to be achieved without causing precipitation of conjugates. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make these fluorophores attractive as fluorescent probes in a variety of fluorescence methods.

 

References
Alkhatatbeh MJ (2011) J Thromb Haemost. 9(4):844. (DyLight 488)
Sarkar P. et al. (2010) J Photochem Photobiol B. 98(1):35. (DyLight 594)

Immunocytochemical labeling of B7-H3 in aldehyde fixed human MCF-7 breast ductal carcinoma cells. The cells were labeled with mouse monoclonal anti-B7-H3/CD276 (BM0451). The antibody was detected using goat anti-mouse DyLight® 650.



The DyLight® 650-conjugated goat polyclonal secondary reagent reacts with the heavy chains of mouse IgG, and the light chains of most mouse immunoglobulins.

Goat polyclonal antibody is supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody has been affinity-purified using mouse IgG coupled to agarose beads. Purified goat polyclonal antibody was conjugated to DyLight® 650 fluorophore (Excitation = 654 nm; Emission = 673 nm). This secondary reagent can be used to detect mouse immunoglobulins in various immunofluorescence applications, such as immunocytochemistry, immunohistochemistry, fluorescent western blot, and flow cytometry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: