Western Blot of lysates from 293 cells either, grown in medium with serum, serum starved, or insulin treated. Using Anti-Phospho-Akt (Ser473) RM251 at a 1:1000 dilution or anti-Akt1 RM252 at a 1:1000 dilution. Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-Akt (Ser473) RM251 at a 1:400 dilution.
Product Details Product Name Anti-Phospho-Akt (Ser473) Rabbit Monoclonal Antibody, Clone RM251 Product Description Rabbit monoclonal to phospho-Akt (Ser473), AKT (phospho S473), AKT1/2/3 (phospho S473) Catalog No. 31-1131-00 Clone Name RM251 Product Data Sheet Anti-Phospho-Akt (Ser473) Rabbit Monoclonal Antibody Data Sheet RM251 Specificity RM251 reacts to Akt only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. This antibody may also react to bovine, mouse or rat Phospho-Akt (Ser473), as predicted by immunogen homology. Immunogen A phospho-peptide corresponding to human Phospho-Akt (Ser473) Application Immunohistochemistry (IHC)
Western Blot (WB)
Species Reactivity Human
(Mouse, Rat, Bovine) Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Immunohistochemistry (IHC): 1:200-1:500 dilution;
Western Blot (WB): 1:1000-1:2000 dilution.
Keywords . Product Images
Anti-Phospho-Akt (Ser473) rabbit monoclonal antibody [RM251]
Cat# 31-1131-00
Size : 100ul
Brand : RevMab Biosciences
Rabbit monoclonal to phospho-Akt (Ser473), AKT (phospho S473), AKT1/2/3 (phospho S473)
100 µL
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