Anti-Phospho-PPP2CA-Tyr307 antibody (260-309 aa)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:5000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PPP2CA
Gene ID:	5515
Uniprot ID:	PP2AA_HUMAN
Immunogen Region:	260-309 aa
Specificity:	Phospho-PP2A-C Alpha (Y307) Polyclonal Antibody detects endogenous levels of PP2A-C Alpha protein only when phosphorylated at Y307.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human PP2A-alpha around the phosphorylation site of Tyr307 at the amino acid range 260-309

Function	PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint. Mediates dephosphorylation of MYC.promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC. Mediates dephosphorylation of FOXO3.promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3. Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome.
Protein Name	Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform Pp2a-Alpha Replication Protein C Rp-C
Database Links	Reactome: R-HSA-113501 Reactome: R-HSA-1295596 Reactome: R-HSA-141444 Reactome: R-HSA-163685 Reactome: R-HSA-163767 Reactome: R-HSA-180024 Reactome: R-HSA-195253 Reactome: R-HSA-196299 Reactome: R-HSA-198753 Reactome: R-HSA-202670 Reactome: R-HSA-2465910 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-2995383 Reactome: R-HSA-389513 Reactome: R-HSA-432142 Reactome: R-HSA-4641262 Reactome: R-HSA-5339716 Reactome: R-HSA-5358747 Reactome: R-HSA-5358749 Reactome: R-HSA-5358751 Reactome: R-HSA-5358752 Reactome: R-HSA-5467337 Reactome: R-HSA-5467340 Reactome: R-HSA-5467348 Reactome: R-HSA-5663220 Reactome: R-HSA-5673000 Reactome: R-HSA-5675221 Reactome: R-HSA-6804757 Reactome: R-HSA-6811558 Reactome: R-HSA-68877 Reactome: R-HSA-69231 Reactome: R-HSA-69273 Reactome: R-HSA-9634600 Reactome: R-HSA-9648025 Reactome: R-HSA-975957
Cellular Localisation	Cytoplasm Nucleus Chromosome Centromere Cytoskeleton Spindle Pole In Prometaphase Cells But Not In Anaphase Cells Localizes At Centromeres During Mitosis Also Found At Spindle Poles Centromeric Localization Requires The Presence Of Sgo2
Alternative Antibody Names	Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform antibody Anti-Pp2a-Alpha antibody Anti-Replication Protein C antibody Anti-Rp-C antibody Anti-PPP2CA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance