Aspartate Transaminase (AST / GOT) Assay Kit is an assay kit designed for the detection and quantification of Aspartate Transaminase activity in serum, plasma, tissue homogenates, cell lysates and cell culture supernatants using a microplate reader (Reitman-Frankel Method). AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish-brown in alkaline solution. Measure the OD values and calculate the enzyme activity. This kit is also available for the Spectrophotometric detection - abx294136.
Research Articles on Aspartate Aminotransferase (AST/GOT)
| Target | Aspartate Aminotransferase (AST/GOT) |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | The validity for this kit is 12 months. |
| Test Range | 1.1 IU/L - 72.3 IU/L |
| Sensitivity | 1.1 IU/L |
| Detection Method | Colorimetric |
| Assay Data | Quantitative |
| CAS Number | 9000-97-9 |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |