Atypical Chemokine Receptor D6 Positive Control

Formulation:	Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	ACKR2
Gene ID:	1238
Uniprot ID:	ACKR2_HUMAN
Specificity:	This is positive control is recommended for use in combination with Atypical Chemokine Receptor D6 antibody STJ500034.

Tissue Specificity	Found in endothelial cells lining afferent lymphatics in dermis and lymph nodes. Also found in lymph nodes subcapsular and medullary sinuses, tonsillar lymphatic sinuses and lymphatics in mucosa and submucosa of small and large intestine and appendix. Also found in some malignant vascular tumors. Expressed at high levels in Kaposi sarcoma-related pathologies. Expressed on apoptotic neutrophils (at protein level). Expressed primarily in placenta and fetal liver, and found at very low levels in the lung and lymph node.
Post Translational Modifications	Phosphorylated on serine residues in the C-terminal cytoplasmic tail.
Function	Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1) through a RAC1-PAK1-LIMK1 signaling pathway. Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. Plays a major role in the immune silencing of macrophages during the resolution of inflammation. Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs.
Peptide Name	Atypical Chemokine Receptor 2 C-C Chemokine Receptor D6 Chemokine Receptor Ccr-10 Chemokine Receptor Ccr-9 Chemokine-Binding Protein 2 Chemokine-Binding Protein D6
Database Links	Reactome: R-HSA-380108
Cellular Localisation	Early Endosome Recycling Endosome Cell Membrane Multi-Pass Membrane Protein Predominantly Localizes To Endocytic Vesicles And Upon Stimulation By The Ligand Is Internalized Via Clathrin-Coated Pits Once Internalized The Ligand Dissociates From The Receptor And Is Targeted To Degradation While The Receptor Is Recycled Back To The Cell Membrane
Alternative Peptide Names	Atypical Chemokine Receptor 2 protein C-C Chemokine Receptor D6 protein Chemokine Receptor Ccr-10 protein Chemokine Receptor Ccr-9 protein Chemokine-Binding Protein 2 protein Chemokine-Binding Protein D6 protein ACKR2 protein CCBP2 protein CCR10 protein CMKBR9 protein D6 protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance