CD45 antibody [2D1] (PerCP-Cy5.5)
Cat# GTX01462-11-100test
Size : 100test
Brand : GeneTex
Contact local distributor :
Phone : +1 850 650 7790
![Anti-CD45 antibody [2D1] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01462-11](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-CD45 antibody [2D1] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01462-11")
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | 2D1 |
Isotype | IgG1 |
Application | FACS |
Reactivity | Human |
| Package | 100 test |
Datasheet File View product citations for antibody GTX01462-11 on CiteAb
PRODUCT
Summary
The 2D1 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, and CD45RO.The 2D1 antibody is widely used as a marker for human CD45 expression on peripheral blood T cells, B cells, monocytes, macrophages, and NK cells.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | 0.125 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test |
Not tested in other applications.
Calculated MW
147 kDa. ( Note )
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.025 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5) Wavelength
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , LCA , LY5 , PTPRC , T200 , protein tyrosine phosphatase, receptor type C , CD45.1 , CD45.2 , CD45RA , CD45RB , CD45RC
Cellular Localization
Membrane,Membrane raft
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Database
DATA IMAGES
| | GTX01462-11 FACS Image FACS analysis of human PBMCs using GTX01462-11 CD45 antibody [2D1] (PerCP-Cy5.5). |
Clone Reference
Zhen Wang et al. Stem Cells Int 2021; 2021:9947187 Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells.
2014; Monocyte-derived dendritic cells from Crohn's disease patients exhibit decreased ability to activate T helper type 17 responses in memory cells.
2010; B cell subsets in healthy children: reference values for evaluation of B cell maturation process in peripheral blood.
1980; Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.
REVIEW
Clone Reference
Zhen Wang et al. Stem Cells Int 2021; 2021:9947187 Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells.
2014; Monocyte-derived dendritic cells from Crohn's disease patients exhibit decreased ability to activate T helper type 17 responses in memory cells.
2010; B cell subsets in healthy children: reference values for evaluation of B cell maturation process in peripheral blood.
1980; Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.
| SDS | |
|---|---|
| Sodium Azide.pdf | |