CF®594 Rabbit Anti-Sheep IgG (H+L), Highly Cross-Adsorbed
Cat# 20173-1mg
Size : 1mg
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
Rabbit Anti-Sheep IgG (H+L), Highly Cross-Adsorbed
Highly cross-adsorbed rabbit anti-sheep IgG (H+L) secondary antibody labeled with our superior CF® dyes.
Technology
CF® Dyes
Considerations for Immunofluorescence Staining
Secondary Antibody Product Listings
Tech Tips
Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Product Description
This is a highly cross-adsorbed rabbit anti-sheep IgG (H L) secondary antibody labeled with our bright and photostable CF® dyes. To minimize cross-reactivity, the antibody has been adsorbed against human serum.
- Highly cross-adsorbed for specific staining with minimal background
- Available in 4 bright and photostable CF® dyes
- Suitable for western, immunofluorescence, and immunohistology in FFPE tissues
Rabbit Anti-Sheep IgG (H+L), Highly Cross-Adsorbed
| Conjugation | Ex/Em | Size | Catalog No. |
|---|---|---|---|
| CF®488A | 490/515 nm | 50 uL (100 ug) | 20172-1 |
| 0.5 mL (1 mg) | 20172 | ||
| 1 mg | 20172-1mg | ||
| CF®543 | 541/560 nm | 50 uL (100 ug) | 20323-1 |
| 0.5 mL (1 mg) | 20323 | ||
| 1 mg | 20323-1mg | ||
| CF®594 | 593/614 nm | 50 uL (100 ug) | 20173-1 |
| 0.5 mL (1 mg) | 20173 | ||
| 1 mg | 20173-1mg | ||
| CF®633 | 630/650 nm | 50 uL (100 ug) | 20174-1 |
| 0.5 mL (1 mg) | 20174 | ||
| 1 mg | 20174-1mg |
View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.
CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.
Storage and Handling
Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.
Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:
CF® Dye and biotin conjugates: add 0.5 mL dH2O
HRP or DNP conjugates: add 1 mL dH2O
Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.
Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.
Note: Storage of the antibody for more than a day at final working dilution is not recommended.
CF is a registered trademark of Biotium, Inc.
References
Download a list of CF® dye references.