DLin-KC2-DMA [1190197-97-7]
Cat# HY-112758-10mg
Size : 10mg
Brand : MedChemExpress
DLin-KC2-DMA is an ionisable cationic lipid (pKa≈6) that is virtually non-toxic to antigen presenting cells (APCs). DLin-KC2-DMA produces significant siRNA-mediated gene silencing of GAPDH, when binds to lipid nanoparticles (LNP). DLin-KC2-DMA can be used in siRNA delivery studies.
For research use only. We do not sell to patients.
DLin-KC2-DMA Chemical Structure
CAS No. : 1190197-97-7
This product is a controlled substance and not for sale in your territory.
Based on 4 publication(s) in Google Scholar
Description |
DLin-KC2-DMA is an ionisable cationic lipid (pKa≈6) that is virtually non-toxic to antigen presenting cells (APCs). DLin-KC2-DMA produces significant siRNA-mediated gene silencing of GAPDH, when binds to lipid nanoparticles (LNP). DLin-KC2-DMA can be used in siRNA delivery studies[1][2]. |
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In Vitro |
DLin-KC2-DMA (1, 5 μg; 72 h) effectively produces a significant siRNA-mediated GAPDH gene silencing in both macrophages and dendritic cells[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cytotoxicity Assay[1]
Western Blot Analysis[1]
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In Vivo |
DLin-KC2-DMA contained-LNP siRNA systems (5 mg/kg; i.v.; single) effectively silences target genes in APcs in vivo[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Molecular Weight |
642.09 |
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Formula |
C43H79NO2 |
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CAS No. |
1190197-97-7 |
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Appearance |
Liquid |
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Color |
Colorless to light yellow |
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SMILES |
CCCCC/C=C\C/C=C\CCCCCCCCC1(CCCCCCCC/C=C\C/C=C\CCCCC)OC(CCN(C)C)CO1 |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
-20°C, stored under nitrogen *The compound is unstable in solutions, freshly prepared is recommended. |
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Solvent & Solubility |
In Vitro:
Ethanol : 100 mg/mL (155.74 mM; Need ultrasonic) DMSO : 100 mg/mL (155.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*The compound is unstable in solutions, freshly prepared is recommended.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.88% |
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References |
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Complete Stock Solution Preparation Table
* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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Ethanol / DMSO | 1 mM | 1.5574 mL | 7.7871 mL | 15.5741 mL | 38.9354 mL |
5 mM | 0.3115 mL | 1.5574 mL | 3.1148 mL | 7.7871 mL | |
10 mM | 0.1557 mL | 0.7787 mL | 1.5574 mL | 3.8935 mL | |
15 mM | 0.1038 mL | 0.5191 mL | 1.0383 mL | 2.5957 mL | |
20 mM | 0.0779 mL | 0.3894 mL | 0.7787 mL | 1.9468 mL | |
25 mM | 0.0623 mL | 0.3115 mL | 0.6230 mL | 1.5574 mL | |
30 mM | 0.0519 mL | 0.2596 mL | 0.5191 mL | 1.2978 mL | |
40 mM | 0.0389 mL | 0.1947 mL | 0.3894 mL | 0.9734 mL | |
50 mM | 0.0311 mL | 0.1557 mL | 0.3115 mL | 0.7787 mL | |
60 mM | 0.0260 mL | 0.1298 mL | 0.2596 mL | 0.6489 mL | |
80 mM | 0.0195 mL | 0.0973 mL | 0.1947 mL | 0.4867 mL | |
100 mM | 0.0156 mL | 0.0779 mL | 0.1557 mL | 0.3894 mL |
DLin-KC2-DMA Related Classifications
- Biochemical Assay Reagents
- Drug Delivery
- Others
- Metabolic Enzyme/Protease
- Liposome