Application
| WB, IHC, ICC, IP, E |
|---|---|
| Primary Accession | Q8NBS9 |
| Other Accession | NP_001139021 |
| Host | Mouse |
| Isotype | IgG2a |
| Reactivity | Human |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human EndoPDI Monoclonal IgG2a |
| Target/Specificity | Detects ~48kDa. |
| Other Names | Thioredoxin domain containing 5; ERP46; UNQ364; MGC3178; FLJ21353; FLJ90810; thioredoxin related protein; endothelial protein disulphide isomeras Antibody |
| Clone Names | 2E7/7 |
| Immunogen | Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-204 was sufficient for detection of EndoPDI in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Endoplasmic Reticulum | Endoplasmic Reticulum Lumen |
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Provided below are standard protocols that you may find useful for product applications.
Background
Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).
References
1. Sullivan D.C., et al. (2003) J Biol Chem. 278 (47) 47079-47088.