Reagents and instruments for immunology, cell biology and molecular biology.
 
> F(ab')2 Anti-Cat IgG F(ab')2 Secondary Antibody

F(ab')2 Anti-Cat IgG F(ab')2 Secondary Antibody

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Cat# ASR3428

Size : 20mg

Brand : Abcepta

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Product Information
Description F(ab')2 Anti-CAT IgG F(ab')2 (RABBIT) Antibody
Host Rabbit
Conjugate Unconjugated
Target Species Cat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Application Note ELISA 1:20,000-1:100,000
Western Blot 1:2,000-1:10,000
Immunohistochemistry 1:1,000-1:5,000
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgG F(ab')2
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Cat IgG F(ab')2 fragment
Reconstitution Volume 2.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer None
Additional Information
Shipping Condition Ambient
Purity This product is a F(ab’)2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgG, Cat IgG F(ab’)2 and Cat Serum.  No reaction was observed against Cat IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.