Specifications

Product Data
Applications	ID, IP
Recommended Dilution	In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgA in Cat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of cat IgA from serum or plasma. Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially) diluted in 2 mm diameter peripheral wells. Antibody titre: 1/32 (Precipitin titre) when tested against pooled normal Cat serum in agar-block immunodiffusion titration.
Reactivities	Feline
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified normal IgA isolated from pooled Cat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is restricted to the Fc part of the IgA molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal cat serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgA. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. The antiserum does not cross react with any other component of the Cat Ig system. Interspecies cross reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross reactivity of this antiserum has not been tested in detail.
Formulation	State: Serum State: Lyophilized (delipidated, heat inactivated) stable whole antiserum without preservatives
Form	Serum
Reconstitution Method	Restore by adding 1 ml of sterile distilled water
Concentration	Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
Conjugation	Unconjugated
Storage	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.