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Immunohistochemistry
Note

Application Data with Unconjugated Antibody

Immunohistochemistry

Immunohistochemical staining of human prostate tissue with FKBP4 monoclonal antibody, clone Hi52C (Cat # MAB11372) shows the strongest staining for FKBP4 is in ductal epithelial cells.

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against synthetic peptide of FKBP4.

    Immunogen

    A synthetic peptide corresponding to residues of human FKBP4.

    Host

    Mouse

    Reactivity

    Dog, Hamster, Human, Mouse, Rat

    Form

    Liquid

    Conjugation

    ATTO 390

    Extinction Coefficient

    24,000

    Excitation (Max)

    390 nm

    Emission (Max)

    479 nm

    Purification

    Protein G purification

    Isotype

    IgG

    Recommend Usage

    Western Blot (1:2000)
    Immunohistochemistry (1:250)
    Immunoprecipitation (5 ug with 10-20 uL Protein A beads)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)

    Storage Instruction

    Store at 4°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    Application Data with Unconjugated Antibody

    Immunohistochemistry

    Immunohistochemical staining of human prostate tissue with FKBP4 monoclonal antibody, clone Hi52C (Cat # MAB11372) shows the strongest staining for FKBP4 is in ductal epithelial cells.

  • Applications

    Western Blot

    Immunohistochemistry

    Immunoprecipitation

  • Gene Info — FKBP4

    Entrez GeneID

    2288

    Protein Accession#

    NP_002005.1

    Gene Name

    FKBP4

    Gene Alias

    FKBP52, FKBP59, HBI, Hsp56, PPIase, p52

    Gene Description

    FK506 binding protein 4, 59kDa

    Omim ID

    600611

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq

    Other Designations

    52 kD FK506 binding protein|FK506 binding protein 4 (59kD)|FK506 binding protein 52|FK506-binding protein 4 (59kD)|HSP binding immunophilin|T-cell FK506-binding protein, 59kD|p59 protein|peptidylprolyl cis-trans isomerase|rotamase

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